Rapid Detection and Differentiation of Clonorchis sinensis and Opisthorchis viverrini Using Real-Time PCR and High Resolution Melting Analysis
Real-time PCR and HRM analysis for all samples. (a) Real-time PCR amplification for all samples. All the heterologous and blank control had no amplification curve before 40 cycles. (b) Melting peaks of liver flukes 1: C. sinensis , 79.05 ± 0.07°C; 2: O. viverrini, , 80.89 ± 0.06°C. (c) Normalized melting curves for two liver flukes.