Bioconversion of Airborne Methylamine by Immobilized Recombinant Amine Oxidase from the Thermotolerant Yeast Hansenula polymorpha

Figure 3

SDS-PAGE in 12% gel of the purified AMO using chromatography on Ni-NTA-agarose. (1) crude extract, 100 𝜇g; (2) flow through the column, 100 𝜇g; (3) washing with 8 mM imidazole, 20 𝜇L; (4) protein molecular weight markers (MW, kDa, 20, 26, 34, 47, 86, and 120; 1.2 𝜇g of each protein); (5)–(14) elution fractions obtained in 10–500 mM imidazole gradient, 20 𝜇L.