The Scientific World Journal / 2014 / Article / Tab 2

Research Article

Two-Component Signal Transduction System SaeRS Positively Regulates Staphylococcus epidermidis Glucose Metabolism

Table 2

Protein expression regulated by saeRS in S. epidermidis.

Spot numberaPredicted MW (Da)Predicted pIProtein hitMowse Scoreb/number of match peptidesAccession numbercFold change (2D: WT/SAE)

Upregulated protein spots
179181244.6PTS system glucose-specific enzyme II A component [Staphylococcus epidermidis ATCC 12228]215/13274680331/4
175181244.6PTS system glucose-specific enzyme II A component [Staphylococcus epidermidis ATCC 12228]156/11274680331/4
21318874.73Accumulation-associated protein [Staphylococcus epidermidis RP62A]88/672428041/10
1211318874.73Accumulation-associated protein [Staphylococcus epidermidis RP62A]190/1672428041/10
171318874.73Accumulation-associated protein [Staphylococcus epidermidis RP62A]72/672428041/10
191318874.73Accumulation-associated protein [Staphylococcus epidermidis RP62A]124/972428041/10
301318874.73Accumulation-associated protein [Staphylococcus epidermidis RP62A]187/1172428041/10
331318874.73Accumulation-associated protein [Staphylococcus epidermidis RP62A]127/772428041/10
11318874.73Accumulation-associated protein [Staphylococcus epidermidis RP62A]83/972428041/10
74350664.72Phosphate acetyltransferase [Staphylococcus epidermidis ATCC 12228]360/19274672771/5
177331315.18Cysteine synthase [Staphylococcus epidermidis ATCC 12228]110/15274691881/2
93332004.75-Oxo-1,2,5-tricarboxylic-3-penten acid decarboxylase [Staphylococcus epidermidis ATCC 12228]83/9274675831/10
222199714.4Heat shock protein 60 [Staphylococcus muscae]81/6172243651/1.5
326153424.74Hypothetical protein SE0591 [Staphylococcus epidermidis ATCC 12228]174/10274675091/2
265186854.5Hypothetical protein SE1560 [Staphylococcus epidermidis ATCC 12228]89/5274684781.5/1

Downregulated protein spots
96316795.35Succinyl-CoA synthetase alpha subunit [Staphylococcus epidermidis ATCC 12228]113/927467842A/Nd
412419984.83Succinyl-CoA synthetase beta subunit [Staphylococcus epidermidis ATCC 12228]80/1127467841A/N
272419984.83Succinyl-CoA synthetase beta subunit [Staphylococcus epidermidis ATCC 12228]74/1427467841A/N
143316795.35Succinyl-CoA synthetase alpha subunit [Staphylococcus epidermidis ATCC 12228]99/10274678427/1
307419984.83Succinyl-CoA synthetase beta subunit [Staphylococcus epidermidis ATCC 12228]134/1927467841A/N
293661964.99D-Fructose-6-phosphate amidotransferase [Staphylococcus epidermidis ATCC 12228]130/1627468669A/N
290349814.66Oxidoreductase ion channel [Staphylococcus epidermidis ATCC 12228]74/9274672856/1
504324905.17Elongation factor Ts [Staphylococcus epidermidis ATCC 12228]159/1727467851A/N
234187486.04Putative vls recombination cassette Vls15 [Borrelia burgdorferi]77/72039294A/N
264364425.29Catabolite control protein A [Staphylococcus epidermidis RP62A]75/957867258A/N
84525895.63Inositol-monophosphate dehydrogenase [Staphylococcus epidermidis ATCC 12228]92/1627469266A/N
260223294.59Hexulose-6-phosphate synthase, putative [Staphylococcus epidermidis RP62A]124/1157866142A/N
386347135.04Glycerate dehydrogenase [Staphylococcus epidermidis RP62A]101/1357867831A/N
754472174.58Enolase [Staphylococcus epidermidis ATCC 12228]84/14274674796/1
194341854.61Putative manganese-dependent inorganic pyrophosphatase [Staphylococcus epidermidis RP62A]190/1957867405A/N
163331315.18Cysteine synthase [Staphylococcus epidermidis ATCC 12228]88/13274691881.5/1
424661964.99D-Fructose-6-phosphate Amidotransferase [Staphylococcus epidermidis ATCC 12228]77/1027468669A/N
171265564.5130S ribosomal protein S1 [Staphylococcus epidermidis ATCC 12228]137/122746808310/1
162420754.62DNA polymerase III Subunit beta [Staphylococcus epidermidis ATCC 12228]79/11274669201.5/1
94629945.24Pyruvate kinase [Staphylococcus epidermidis ATCC 12228]230/2027468291A/N
415465905.01Isocitrate dehydrogenase [Staphylococcus epidermidis ATCC 12228]72/7274682887/1
82661054.57DnaK protein [Staphylococcus epidermidis RP62A]257/17578670375/1
96661054.57DnaK protein [Staphylococcus epidermidis RP62A]77/7578670375/1
72661054.57DnaK protein [Staphylococcus epidermidis RP62A]84/10578670375/1
21661054.57DnaK protein [Staphylococcus epidermidis RP62A]157/12578670375/1
74661054.57DnaK protein [Staphylococcus epidermidis RP62A]88/1457867037A/N
142330314.82Fructose-bisphosphate aldolase [Staphylococcus epidermidis ATCC 12228]261/24274690743/1
481149065.96Urease beta subunit [Staphylococcus epidermidis ATCC 12228]218/18274687804/1
90361684.83Glyceraldehyde-3-phosphate dehydrogenase [Staphylococcus epidermidis ATCC 12228]123/1327467475A/N
83525895.63Inositol-monophosphate dehydrogenase [Staphylococcus epidermidis ATCC 12228]86/1327469266A/N
45525895.63Inositol-monophosphate dehydrogenase [Staphylococcus epidermidis ATCC 12228]106/1627469266A/N
44525895.63Inositol-monophosphate dehydrogenase [Staphylococcus epidermidis ATCC 12228]205/2727469266A/N
101487024.28Trigger factor [Staphylococcus epidermidis ATCC 12228]89/13274682683/1
148515244.62ATP synthase subunit B [Staphylococcus epidermidis ATCC 12228]96/6274686184/1
148515244.62ATP synthase subunit B [Staphylococcus epidermidis ATCC 12228]107/7274686184/1
227202035.71Immunodominant antigen B [Staphylococcus epidermidis ATCC 12228]81/8274684931.5/1
237351515.42Hypothetical protein SE1260 [Staphylococcus epidermidis ATCC 12228]112/16274681784/1
261351515.42Hypothetical protein SE1260 [Staphylococcus epidermidis ATCC 12228]111/16274681781.5/1
654177535.55Hypothetical protein SE2391 [Staphylococcus epidermidis ATCC 12228]126/102746930935/1
320191824.69Hypothetical protein SE0264 [Staphylococcus epidermidis ATCC 12228]113/152746718210/1

Spots numbers correspond to the numbers in Figure 2.
bProtein score was from MALDI-TOF/TOF identification. The proteins that had a score great than 72 ( ) were considered statistically significant.
cAccession no. is the MASCOT result of MALDI-TOF/TOF searched from the NCBInr database.
dA/N indicates the spot detected only in the protein extracts of S. epidermidis 1457 wild-type strain.