Table of Contents Author Guidelines Submit a Manuscript
The Scientific World Journal
Volume 2014, Article ID 921976, 10 pages
http://dx.doi.org/10.1155/2014/921976
Research Article

rre37 Overexpression Alters Gene Expression Related to the Tricarboxylic Acid Cycle and Pyruvate Metabolism in Synechocystis sp. PCC 6803

RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan

Received 4 September 2014; Revised 22 November 2014; Accepted 23 November 2014; Published 28 December 2014

Academic Editor: Anli Geng

Copyright © 2014 Hiroko Iijima et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. T. Kaneko, S. Sato, H. Kotani et al., “Sequence analysis of the genome of the unicellular cyanobacterium synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions,” DNA Research, vol. 3, no. 3, pp. 109–136, 1996. View at Publisher · View at Google Scholar · View at Scopus
  2. S. Zhang and D. A. Bryant, “The tricarboxylic acid cycle in cyanobacteria,” Science, vol. 334, no. 6062, pp. 1551–1553, 2011. View at Publisher · View at Google Scholar · View at Scopus
  3. M. I. Muro-Pastor, J. C. Reyes, and F. J. Florencio, “Cyanobacteria perceive nitrogen status by sensing intracellular 2-oxoglutarate levels,” The Journal of Biological Chemistry, vol. 276, no. 41, pp. 38320–38328, 2001. View at Google Scholar · View at Scopus
  4. R. Tanigawa, M. Shirokane, S.-I. Maeda, T. Omata, K. Tanaka, and H. Takahashi, “Transcriptional activation of NtcA-dependent promoters of Synechococcus sp. PCC 7942 by 2-oxoglutarate in vitro,” Proceedings of the National Academy of Sciences of the United States of America, vol. 99, no. 7, pp. 4251–4255, 2002. View at Publisher · View at Google Scholar · View at Scopus
  5. M. F. Vázquez-Bermúdez, A. Herrero, and E. Flores, “2-oxoglutarate increases the binding affinity of the NtcA (nitrogen control) transcription factor for the Synechococcus glnA promoter,” FEBS Letters, vol. 512, no. 1–3, pp. 71–74, 2002. View at Publisher · View at Google Scholar · View at Scopus
  6. O. Fokina, V.-R. Chellamuthu, K. Forchhammer, and K. Zeth, “Mechanism of 2-oxoglutarate signaling by the Synechococcus elongatus PII signal transduction protein,” Proceedings of the National Academy of Sciences of the United States of America, vol. 107, no. 46, pp. 19760–19765, 2010. View at Publisher · View at Google Scholar · View at Scopus
  7. K. Zeth, O. Fokinas, and K. Forchhammers, “Structural basis and target-specific modulation of ADP sensing by the Synechococcus elongatus PII signaling protein,” Journal of Biological Chemistry, vol. 289, no. 13, pp. 8960–8972, 2014. View at Publisher · View at Google Scholar · View at Scopus
  8. T. Hasunuma, F. Kikuyama, M. Matsuda, S. Aikawa, Y. Izumi, and A. Kondo, “Dynamic metabolic profiling of cyanobacterial glycogen biosynthesis under conditions of nitrate depletion,” Journal of Experimental Botany, vol. 64, no. 10, pp. 2943–2954, 2013. View at Publisher · View at Google Scholar · View at Scopus
  9. W. Hauf, M. Schlebusch, J. Hüge, J. Kopka, M. Hagemann, and K. Forchhammer, “Metabolic changes in Synechocystis PCC6803 upon nitrogen-starvation: excess NADPH sustains polyhydroxybutyrate accumulation,” Metabolites, vol. 3, no. 1, pp. 101–118, 2013. View at Publisher · View at Google Scholar
  10. T. Osanai, A. Oikawa, T. Shirai et al., “Capillary electrophoresis-mass spectrometry reveals the distribution of carbon metabolites during nitrogen starvation in Synechocystis sp. PCC 6803,” Environmental Microbiology, vol. 16, no. 2, pp. 512–524, 2014. View at Publisher · View at Google Scholar · View at Scopus
  11. S. Meissner, D. Steinhauser, and E. Dittmann, “Metabolomic analysis indicates a pivotal role of the hepatotoxin microcystin in high light adaptation of Microcystis,” Environmental Microbiology, 2014. View at Publisher · View at Google Scholar
  12. T. Osanai, S. Imamura, M. Asayama et al., “Nitrogen induction of sugar catabolic gene expression in Synechocystis sp. PCC 6803,” DNA Research, vol. 13, no. 5, pp. 185–195, 2006. View at Publisher · View at Google Scholar · View at Scopus
  13. S. Ehira and M. Ohmori, “NrrA, a nitrogen-responsive response regulator facilitates heterocyst development in the cyanobacterium Anabaena sp. strain PCC 7120,” Molecular Microbiology, vol. 59, no. 6, pp. 1692–1703, 2006. View at Publisher · View at Google Scholar · View at Scopus
  14. S. Ehira and M. Ohmori, “NrrA directly regulates expression of hetR during heterocyst differentiation in the cyanobacterium Anabaena sp. strain PCC 7120,” Journal of Bacteriology, vol. 188, no. 24, pp. 8520–8525, 2006. View at Publisher · View at Google Scholar · View at Scopus
  15. S. Ehira and M. Ohmori, “NrrA, a nitrogen-regulated response regulator protein, controls glycogen catabolism in the nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120,” The Journal of Biological Chemistry, vol. 286, no. 44, pp. 38109–38114, 2011. View at Publisher · View at Google Scholar · View at Scopus
  16. Y. Tabei, K. Okada, and M. Tsuzuki, “Sll1330 controls the expression of glycolytic genes in Synechocystis sp. PCC 6803,” Biochemical and Biophysical Research Communications, vol. 355, no. 4, pp. 1045–1050, 2007. View at Publisher · View at Google Scholar · View at Scopus
  17. M. Azuma, T. Osanai, M. Y. Hirai, and K. Tanaka, “A response regulator Rre37 and an RNA polymerase sigma factor SigE represent two parallel pathways to activate sugar catabolism in a cyanobacterium Synechocystis sp. PCC 6803,” Plant and Cell Physiology, vol. 52, no. 2, pp. 404–412, 2011. View at Publisher · View at Google Scholar · View at Scopus
  18. D. Liu and C. Yang, “The nitrogen-regulated response regulator nrra controls cyanophycin synthesis and glycogen catabolism in the cyanobacterium Synechocystis sp. PCC 6803,” The Journal of Biological Chemistry, vol. 289, no. 4, pp. 2055–2071, 2014. View at Publisher · View at Google Scholar · View at Scopus
  19. T. Osanai, A. Oikawa, K. Numata et al., “Pathway-level acceleration of glycogen catabolism by a response regulator in the cyanobacterium Synechocystis species PCC 6803,” Plant Physiology, vol. 164, no. 4, pp. 1831–1841, 2014. View at Publisher · View at Google Scholar · View at Scopus
  20. A. Joseph, S. Aikawa, K. Sasaki et al., “Rre37 stimulates accumulation of 2-oxoglutarate and glycogen under nitrogen starvation in Synechocystis sp. PCC 6803,” FEBS Letters, vol. 588, no. 3, pp. 466–471, 2014. View at Publisher · View at Google Scholar · View at Scopus
  21. J. G. K. Williams, “Construction of specific mutations in photosystem II photosynthetic reaction center by genetic engineering methods in Synechocystis 6803,” Methods in Enzymology, vol. 167, pp. 766–778, 1988. View at Publisher · View at Google Scholar · View at Scopus
  22. R. Rippka, “Isolation and purification of cyanobacteria,” Methods in Enzymology, vol. 167, pp. 3–27, 1988. View at Publisher · View at Google Scholar · View at Scopus
  23. Y. Kanesaki, Y. Shiwa, N. Tajima et al., “Identification of substrain-specific mutations by massively parallel whole-genome resequencing of Synechocystis sp. PCC 6803,” DNA Research, vol. 19, no. 1, pp. 67–79, 2012. View at Publisher · View at Google Scholar · View at Scopus
  24. M. Isabel Muro-Pastor, J. C. Reyes, and F. J. Florencio, “The NADP+-isocitrate dehydrogenase gene (icd) is nitrogen regulated in cyanobacteria,” Journal of Bacteriology, vol. 178, no. 14, pp. 4070–4076, 1996. View at Google Scholar · View at Scopus
  25. Z. Su, V. Olman, F. Mao, and Y. Xu, “Comparative genomics analysis of NtcA regulons in cyanobacteria: regulation of nitrogen assimilation and its coupling to photosynthesis,” Nucleic Acids Research, vol. 33, no. 16, pp. 5156–5171, 2005. View at Publisher · View at Google Scholar · View at Scopus
  26. S. Picossi, E. Flores, and A. Herrero, “ChIP analysis unravels an exceptionally wide distribution of DNA binding sites for the NtcA transcription factor in a heterocyst-forming cyanobacterium,” BMC Genomics, vol. 15, no. 1, article 22, 2014. View at Publisher · View at Google Scholar · View at Scopus
  27. T. Osanai, M. Imashimizu, A. Seki et al., “ChlH, the H subunit of the Mg-chelatase, is an anti-sigma factor for SigE in Synechocystis sp. PCC 6803,” Proceedings of the National Academy of Sciences of the United States of America, vol. 106, no. 16, pp. 6860–6865, 2009. View at Publisher · View at Google Scholar · View at Scopus
  28. T. Osanai, A. Oikawa, M. Azuma et al., “Genetic engineering of group 2 σ factor SigE widely activates expressions of sugar catabolic genes in Synechocystis species PCC 6803,” The Journal of Biological Chemistry, vol. 286, no. 35, pp. 30962–30971, 2011. View at Publisher · View at Google Scholar · View at Scopus
  29. T. Osanai, K. Numata, A. Oikawa et al., “Increased bioplastic production with an RNA polymerase sigma factor SigE during nitrogen starvation in Synechocystis sp. PCC 6803,” DNA Research, vol. 20, no. 6, pp. 525–535, 2013. View at Publisher · View at Google Scholar · View at Scopus
  30. T. Osanai, A. Kuwahara, H. Iijima et al., “Pleiotropic effect of sigE over-expression on cell morphology, photosynthesis and hydrogen production in Synechocystis sp. PCC 6803,” The Plant Journal, vol. 76, no. 3, pp. 456–465, 2013. View at Publisher · View at Google Scholar · View at Scopus
  31. Y. Kaniya, A. Kizawa, A. Miyagi et al., “Deletion of the transcriptional regulator cyAbrB2 deregulates primary carbon metabolism in Synechocystis sp. PCC 6803,” Plant Physiology, vol. 162, no. 2, pp. 1153–1163, 2013. View at Publisher · View at Google Scholar · View at Scopus
  32. S. Imamura, K. Tanaka, M. Shirai, and M. Asayama, “Growth phase-dependent activation of nitrogen-related genes by a control network of group 1 and group 2 σ factors in a cyanobacterium,” Journal of Biological Chemistry, vol. 281, no. 5, pp. 2668–2675, 2006. View at Publisher · View at Google Scholar · View at Scopus
  33. J. Espinosa, K. Forchhammer, S. Burillo, and A. Contreras, “Interaction network in cyanobacterial nitrogen regulation: PipX, a protein that interacts in a 2-oxoglutarate dependent manner with PII and NtcA,” Molecular Microbiology, vol. 61, no. 2, pp. 457–469, 2006. View at Publisher · View at Google Scholar · View at Scopus
  34. R. López-Igual, S. Picossi, J. López-Garrido, E. Flores, and A. Herrero, “N and C control of ABC-type bicarbonate transporter Cmp and its LysR-type transcriptional regulator CmpR in a heterocyst-forming cyanobacterium, Anabaena sp,” Environmental Microbiology, vol. 14, no. 4, pp. 1035–1048, 2012. View at Publisher · View at Google Scholar · View at Scopus
  35. N. C. Bhatt, A. Panwar, T. S. Bisht, and S. Tamta, “Coupling of algal biofuel production with wastewater,” The Scientific World Journal, vol. 2014, Article ID 210504, 10 pages, 2014. View at Publisher · View at Google Scholar
  36. B. A. Neilan, L. A. Pearson, J. Muenchhoff, M. C. Moffitt, and E. Dittmann, “Environmental conditions that influence toxin biosynthesis in cyanobacteria,” Environmental Microbiology, vol. 15, no. 5, pp. 1239–1253, 2013. View at Publisher · View at Google Scholar · View at Scopus