Parameter Screening in Microfluidics Based Hydrodynamic Single-Cell Trapping
Figure 1
Principle of single-cell positioning based on the hydrodynamic trapping mechanism. (a) Schematic of the cell trapping structure, which consists of two parallel channels (main channel and buffer channel) connected with cellular trapping sites with a decrease in the cross-sectional area. A, B, C, and D represent the main channel inlet, main channel outlet, buffer channel inlet, and buffer channel outlet, respectively. Four critical geometry parameters of the trapping site , , , and were illustrated in (b). For each channel inlet or outlet, there are three possible statuses, which are I (inlet with a defined fluid flow rate as boundary condition), O (outlet with zero pressure as boundary condition), and W (wall with zero fluid flow velocity as boundary condition). By regulating the status of channel inlets and outlets, this structure can represent previous designs where (c) represents the structure proposed by Shoji et al. [26] and (d) presents the structure put forward by Lu et al. [24].
