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The Scientific World Journal
Volume 2014, Article ID 938272, 10 pages
Research Article

Synthesis and Characterization of Silver and Gold Nanoparticles Using Aqueous Extract of Seaweed, Turbinaria conoides, and Their Antimicrofouling Activity

1Department of Oceanography and Coastal Area Studies, School of Marine Sciences, Alagappa University, Thondi Campus, Thondi, Tamil Nadu 623 409, India
2SRM Research Institute, SRM University, Kattankulathur, Kancheepuram, Tamil Nadu 603 203, India
3Department of Nanoscience and Technology, Alagappa University, Karaikudi, Tamil Nadu 630 003, India
4Department of Zoology, Directorate of Distance Education, Alagappa University, Karaikudi, Tamil Nadu 630 003, India
5Biological Oceanography Division, CSIR-National Institute of Oceanography, Dona Paula, Goa 403 004, India

Received 9 August 2013; Accepted 5 December 2013; Published 3 February 2014

Academic Editors: C. Boyer, A. M. Noreddin, M. Rai, and P. Webster

Copyright © 2014 Sri Ramkumar Vijayan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Silver and gold nanoparticles were synthesized using an aqueous extract of the seaweed Turbinaria conoides and their antibiofilm activity against marine biofilm forming bacteria is reported here. The UV-Vis spectra showed the characteristics SPR absorption band for Ag NPs at 421 and for Au NPs at 538 nm. Further, the synthesized nanoparticles were characterized using FT-IR, XRD, FESEM, EDX, and HRTEM analysis. Spherical and triangular nanostructures of the Ag and Au nanoparticles were observed between the size ranges of 2–17 nm and 2–19 nm, respectively. The synthesized Ag NPs are efficient in controlling the bacterial biofilm formation; however, Au NPs did not show any remarkable antibiofilm activity. The maximum zone of inhibition was recorded against E. coli (  mm), followed by Salmonella sp., S. liquefaciens, and A. hydrophila. The macrotube dilution method inferred the MIC (20–40 µL mL−1) and MBC (40–60 µL mL−1) of Ag NPs. The CLSM images clearly showed the weak adherence and disintegrating biofilm formation of marine biofilm bacterial strains treated with Ag NPs. The Artemia cytotoxicity assay recorded the LC50 value of µL mL−1. Thus the present study proved the efficiency of Ag NPs as a potent antimicrofouling agent and became the future perspective for the possible usage in the biofouling related issues in the aquaculture installations and other marine systems.