miR-16-1 Promotes the Aberrant α-Synuclein Accumulation in Parkinson Disease via Targeting Heat Shock Protein 70

Figure 2

Blockage of Hsp70 by chemicals promotes α-synuclein aggregation in SH-SY5Y-Syn cells. (a) Relative Hsp70 expression to β-actin in mRNA level, in SH-SY5Y-Syn cells after MAL3-101 treatment or Hsp70 silence by siRNA, revealing by RT-qPCR. (b) Relative Hsp70 expression to β-actin in protein level, in SH-SY5Y-Syn cells after MAL3-101 treatment or Hsp70 silence by siRNA, revealing by western blot analysis. (c) Blockage of Hsp70 by chemicals or by RNAi technology promotes α-synuclein aggregation in SH-SY5Y-Syn cells. All results were got from triplicate independent experiments. ^∗𝑃<0.05, ^∗∗𝑃<0.01, ns: no significance.

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