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The Scientific World Journal
Volume 2014, Article ID 943825, 8 pages
http://dx.doi.org/10.1155/2014/943825
Research Article

Physical Mapping of the 5S and 18S rDNA in Ten Species of Hypostomus Lacépède 1803 (Siluriformes: Loricariidae): Evolutionary Tendencies in the Genus

1Universidade Tecnológica Federal do Paraná, Prolongamento da Rua Cerejeira s/n, São Luiz, 85892-00l Santa Helena, PR, Brazil
2Departamento de Biologia e Zoologia, Universidade Federal de Mato Grosso, Instituto de Biociências, Avenida Fernando Correa da Costa s/n, Coxipó, 78060-900 Cuiabá, MT, Brazil
3Universidade Estadual do Oeste do Paraná, Centro de Ciências Biológicas e da Saúde, Rua Universitária 2069, 85801-110 Cascavel, PR, Brazil
4Departamento de Biologia/Nupélia, Universidade Estadual de Maringá, Avenida Colombo 5790, 87020-900 Maringá, PR, Brazil
5Departamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta Grossa, Avenida Carlos Cavalcanti 4748, 84030-900 Ponta Grossa, PR, Brazil

Received 30 July 2014; Accepted 24 September 2014; Published 27 October 2014

Academic Editor: Adayabalam S. Balajee

Copyright © 2014 Vanessa Bueno et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Hypostomus is a diverse group with unclear aspects regarding its biology, including the mechanisms that led to chromosome diversification within the group. Fluorescence in situ hybridization (FISH) with 5S and 18S rDNA probes was performed on ten Hypostomini species. Hypostomus faveolus, H. cochliodon, H. albopunctatus, H. aff. paulinus, and H. topavae had only one chromosome pair with 18S rDNA sites, while H. ancistroides, H. commersoni, H. hermanni, H. regani, and H. strigaticeps had multiple 18S rDNA sites. Regarding the 5S rDNA genes, H. ancistroides, H. regani, H. albopunctatus, H. aff. paulinus, and H. topavae had 5S rDNA sites on only one chromosome pair and H. faveolus, H. cochliodon, H. commersoni, H. hermanni, and H. strigaticeps had multiple 5S rDNA sites. Most species had 18S rDNA sites in the telomeric region of the chromosomes. All species but H. cochliodon had 5S rDNA in the centromeric/pericentromeric region of one metacentric pair. Obtained results are discussed based on existent phylogenies for the genus, with comments on possible dispersion mechanisms to justify the variability of the rDNA sites in Hypostomus.