Dietary Conjugated Linoleic Acid Alters Oxidative Stability and Alleviates Plasma Cholesterol Content in Meat of Broiler Chickens

Table 1

Ingredients and chemical compositions of experimental diets.

Ingredient (% DM)	Experimental diets
	Starter (1–21 days)			Finisher (22–42 days)
	CON	LCLA	HCLA	CON	LCLA	HCLA
Corn	51.17	51.17	51.17	58.9	58.9	58.9
Soybean meal	40.56	40.56	40.56	32.22	32.22	32.22
Palm oil	5	2.5	—	5	2.5	—
Lutrell	—	2.5	5	—	2.5	5
DiCalcalcium phosphate	1.34	1.34	1.34	1.4	1.4	1.4
Limestone	1.7	1.7	1.7	1.19	1.19	1.19
Common salt	0.4	0.4	0.4	0.4	0.4	0.4
^1Vitamin premix	0.05	0.05	0.05	0.05	0.05	0.05
^1Mineral premix	0.05	0.05	0.05	0.05	0.05	0.05
DL-methionine	0.26	0.26	0.26	0.3	0.3	0.3
Lysine	—	—	—	0.5	0.5	0.5
^2Chemical composition
Crude protein (% DM)	22.00	22.00	22.00	20.5	20.5	20.5
Metabolizable energy (Kcal/kg)	3080	3080	3080	3150	3150	3150
Crude fat (%)	7.27	7.27	7.27	7.50	7.50	7.50
Phosphorus (% DM)	0.45	0.45	0.45	0.42	0.42	0.42
Calcium (% DM)	1.00	1.00	1.00	0.9	0.9	0.9
Methionine (% DM)	0.55	0.55	0.55	0.5	0.5	0.5
Lysine (% DM)	1.20	1.20	1.20	1	1	1
Na (% DM)	0.20	0.20	0.20	0.15	0.15	0.15

CON: CON; LCLA: low conjugated linoleic acid; HCLA: high conjugated linoleic acid.
¹Premixes contributed the following nutrients per kilogram of complete feed: vitamin A, 2300 IU; vitamin D₃, 400 IU; vitamin E, 1.8 mg; vitamin B₁₂, 3.5 mg; riboflavin, 1.4 mg; pantothenic acid, 2 mg; nicotinic acid, 7 mg; pyridoxine, 0.25 mg; folic acid, 0.15 mg; menadione, 0.3 mg; thiamin, 0.15 mg; manganese oxide, 35 mg; ferrous sulfate 35 mg; zinc oxide, 30 mg; copper sulfate, 60 mg; cobalt carbonate, 5 mg; potassium iodine, 0.6 mg; selenium vanadate, 0.09 mg. CLA used in this study was Lutrell pure, BASF, Germany, which contained 60% CLA. Dietary inclusion of 5% and 2.5% Lutrell will be 1.5% and 3% of both CLA isomers, respectively.
²Calculated values.