Research Article
A Broad-Spectrum Antimicrobial Activity of Bacillus subtilis RLID 12.1
Table 4
Summary of purification steps.
| | Purification steps | Total
Protein
(mg) | Total
Activity
(AU) | Specific
activitya | Purification fold | Yield, % |
| Cell-free
supernatant (CFS) | 13440 | 24000 | 1.785 | 1 | 100 | | Ultrafiltration | 1000 | 16000 | 16 | 8.9 | 66.7 | Ammonium
sulphate
precipitation (30%) | 80.1 | 9600 | 119.85 | 35.85 | 40 | DEAE-Sepharose
chromatography | 20 | 6400 | 320 | 179.27 | 26 |
|
|
aSpecific activity is the ratio of total activity to that of total protein.
|
