The Scientific World Journal / 2014 / Article / Tab 2

Research Article

Enhanced Production and Characterization of a Solvent Stable Amylase from Solvent Tolerant Bacillus tequilensis RG-01: Thermostable and Surfactant Resistant

Table 2

Stability of crude amylase in presence of various organic solvents.

Organic solvents (30%)Residual activity (%)
1 h24 h48 h72 h96 h120 h144 h168 h

Methanol−0.76101.9130.5133.2125.3120.6113.4106.393.3
Isopropanol−0.2890.2100.396.390.489.586.480.375.4
Ethanol−0.2497105.6103.5100.696.590.481.278.3
Benzene2.1399110959086817872
Cyclohexane3.38710512010299918075
Acetone−0.23101.5105.4103.9100.895908580
Butanol−0.80112.7144.3128.2115.4107.6100.310095
Toluene2.5115.9170.2140.1127.1120.8112.9105.5100.3
Isooctane2.9120160.1125.5120.5113.4110.3105.799.2
Xylene3.1891071201101061009491
Hexane3.6114133124118113104100100
n-Decane5.6125.3151.3168.8148.3126.2101.710099.0
n-Dodecane6.0131.8160.2232.3158.1149.9120.2108.0100.0

Enzyme was preincubated with different organic solvents at a concentration of 30% (v/v) at 55°C for different time periods and assayed as standard assay method. The enzyme activity without incubation with organic solvent was taken as 100%. Mean standard deviation for all values is <±5.0%.

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