Enhanced Production and Characterization of a Solvent Stable Amylase from Solvent Tolerant Bacillus tequilensis RG-01: Thermostable and Surfactant Resistant
Table 2
Stability of crude amylase in presence of various organic solvents.
Organic solvents (30%)
Residual activity (%)
1 h
24 h
48 h
72 h
96 h
120 h
144 h
168 h
Methanol
−0.76
101.9
130.5
133.2
125.3
120.6
113.4
106.3
93.3
Isopropanol
−0.28
90.2
100.3
96.3
90.4
89.5
86.4
80.3
75.4
Ethanol
−0.24
97
105.6
103.5
100.6
96.5
90.4
81.2
78.3
Benzene
2.13
99
110
95
90
86
81
78
72
Cyclohexane
3.3
87
105
120
102
99
91
80
75
Acetone
−0.23
101.5
105.4
103.9
100.8
95
90
85
80
Butanol
−0.80
112.7
144.3
128.2
115.4
107.6
100.3
100
95
Toluene
2.5
115.9
170.2
140.1
127.1
120.8
112.9
105.5
100.3
Isooctane
2.9
120
160.1
125.5
120.5
113.4
110.3
105.7
99.2
Xylene
3.1
89
107
120
110
106
100
94
91
Hexane
3.6
114
133
124
118
113
104
100
100
n-Decane
5.6
125.3
151.3
168.8
148.3
126.2
101.7
100
99.0
n-Dodecane
6.0
131.8
160.2
232.3
158.1
149.9
120.2
108.0
100.0
Enzyme was preincubated with different organic solvents at a concentration of 30% (v/v) at 55°C for different time periods and assayed as standard assay method. The enzyme activity without incubation with organic solvent was taken as 100%. Mean standard deviation for all values is <±5.0%.