Enzymatic Activity Analysis and Catalytic Essential Residues Identification of Brucella abortus Malate Dehydrogenase

Figure 6

Sequence comparisons and bioinformatics analysis. Alignment of the amino acid sequences of MDH from E. coli K-12 (access number P61889), S. enterica (access number YP_002245258), T. maritime (access number P16115), A. fulgidus (access number Z85985), and B. abortus (access number NC_007618.1) by DNAstar 7.01 version. Gaps are marked by dots. Residues important for catalysis, coenzyme binding, and substrate binding are indicated by asterisks and numbered according to the system used for lactate dehydrogenase.