Identification, Cloning, and Expression of L-Amino Acid Oxidase from Marine Pseudoalteromonas sp. B3

Table 2

Conserved regions of LAAOs from Pseudoalteromonas microorganisms used for design of degenerate primers.

Item	Accession no.	Sequences of^a
		Region 1	Region 2	Region 3
Pseudoalteromonas haloplanktis TAC125	YP_339251	13IIGSGAAAGLS22	225AMAWRAGC232	359MTDFNGKTDL368
Pseudoalteromonas tunicata D2	ZP_01132853	13IIGSGAAAGLS22	224AMAWRAGC231	357MTDFNGKTDL366
Pseudoalteromonas atlantica T6c	YP_662716	13IIGSGAAAGLS22	222AMAWRAGC230	359VTDFNAKTDL368
Pseudoalteromonas sp. SM9913	YP_004069407	13IIGSGAAAGLS22	225AMAWRAGC232	359MTDFNGKTDL368
Degenerate primer^b		ZZ-2: 5′-ATTATHGGYAGCGGCGC-3′	ZZ-3: 5′-GCHATGGCDTGGCGTGC-3′; ZF-2: 5′-CCHGCACGCCAHGCCAT-3′	ZF-3: 5′-TDSCATTAAAGTCRGTC-3′

aBoldface letters indicate the specific amino acids actually used in the design of the degenerate primers (forward primers: ZZ-2 and ZZ-3; reverse primers: ZF-2 and ZF-3).