Identification, Cloning, and Expression of L-Amino Acid Oxidase from Marine Pseudoalteromonas sp. B3

Table 3

Primers used in cloning of 5′ and 3′ flanked sequences of LAAO gene from Pseudoalteromonas sp. B3.

Primers*	Sequences (5′-3′)	Size (bp)
Set A
5F-1	TTGCCAAAGACAGAGCCAGGCT	22
5F-2	AGGTCGCCCTTACTGAGCACTGT	23
5F-3	TCTTATCAAACACCGCAGCAAT	22
Set B
3Z-1	ATTGCCATGGCTTGGCGTG	18
3Z-2	GTCAAATCCAGACGTTTCCAGT	22
3Z-3	GCGGTGCCAGTAAAGTGTATCT	22
Set C
S-1	ACGATGGACTCCAGAGCGGCCCGCVNVNNNGGAA	34
S-2	ACGATGGACTCCAGAGCGGCCCGCBNBNNNGGTT	34
S-3	ACGATGGACTCCAGAGCGGCCCGCHNVNNNCCAC	34
S-4	ACGATGGACTCCAGAGCGGCCCGCVVNVNNNCCAA	35
S-5	ACGATGGACTCCAGAGCGGCCCGCBDNBNNNCGGT	35
B0043	NNNNNN	6
B0043-9	NNNNNNNNN	9
B0043-10	NNNNNNNNNN	10

∗Primers in set A are one set of nested specific primers for amplification of 5′ flanked region of B3-LAAO gene; primers in set B are one set of nested primers for amplification of 3′ flanked region of B3-LAAO gene; primers in set C are arbitrary primers for amplification of 5′ and 3′ flanked regions of B3-LAAO gene.