freeQuant: A Mass Spectrometry Label-Free Quantification Software Tool for Complex Proteome Analysis

Figure 6

The quantitative analysis of ETC complex subunits. Three groups of proteins with different complexes were compared with averaged NSAF of each ETC complex. (a) shows quantitative abundance of all five ETC complexes; Complex I to Complex IV displayed very similar level of abundances. In comparison, Complex V appeared to be in higher abundance, approximately 3-fold more than others. (b) shows quantitative analysis of proteins in Complex I and proteins were encoded by mitochondrial genome or nuclear genome, respectively; (c) shows quantitative analysis of proteins in Complex II; (d) shows quantitative analysis of proteins in Complex III and proteins were encoded by mitochondrial genome or nuclear genome, respectively; (e) shows quantitative analysis of proteins in Complex IV and proteins were encoded by mitochondrial genome or nuclear genome, respectively; (f) shows quantitative analysis of proteins in Complex V and proteins were encoded by mitochondrial genome or nuclear genome, respectively.

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