Research Article
Mechanism of Gene Amplification via Yeast Autonomously Replicating Sequences
Table 3
Primers used for the PCR amplification of APS used in this study.
| APS | Primer | Primer sequence (5′-3′) | Product size (bp) | (°C) |
| ARS315 | 315 F | GGGATACGACCGGTCCTGAGAAA | 428 | 56 | ARS315 | 315 R | CCGCAAAGATGTGCGGGAAATTCT | 428 | 56 | ARS606 | 606 F | GGTCTTCTTGATAATTCTGTGGGCGCT | 367 | 57 | ARS606 | 606 R | GGTACCATATGAAACTCCAGCAGC | 367 | 57 | ARS1305 | 1305 F | TGGCCATTGCGTTGTCATTGAGTG | 281 | 59 | ARS1305 | 1305 R | GGGTGGAGTAATGGGTTTCACAAAGG | 281 | 59 | ARS1426 | 1426 F | ACCGTAGCCGTAACGTTTAATGCC | 328 | 57 | ARS1426 | 1426 R | CGAGGAGATCCTCTTCTCTTTGCT | 328 | 57 | ARS1512 | 1512 F | TCCAATGGCTTAGTAGCTGCCAGA | 381 | 59 | ARS1512 | 1512 R | TGACAACAAGGAGGACAGCGAACA | 381 | 59 | PO5 | PO5 F | GGGTGCGATCATACAGCGT | 320 | 49 | PO5 | PO5 R | GGGTGCCAACACTAGGACTTC | 320 | 49 | PL5 | PL5 F | GAGTTCTGATGGGATCCGGTG | 350 | 51 | PL5 | PL5 R | CGCTTGGGCTAGAGCAGTAC | 350 | 51 | Ste20-YF1 | YF1F | AAATGTCCAAATATACACGATAAG | 613 | 54 | Ste20-YF1 | YF2R | AGAACTACTATGGTGGGAACG | 613 | 54 | Ste20-YF2 | YF2F | TGTGTTGTCAGCAGAATGATCCGGG | 529 | 53 | Ste20-YF2 | YF2R | CGAGGTAGCAAGCAACCCAAACTT | 529 | 53 | Ste20-YF3 | YF3F | ATGAAGCAATTCCGTAGCGCTTGC | 531 | 54 | Ste20-YF3 | YF3R | AGGCGACCTTTGGGTCATTATGGA | 531 | 54 | Ste20-YF4 | YF4F | TGCTCGTTGTCGTTATCGTCGTCA | 427 | 57 | Ste20-YF4 | YF4R | AGAACTACTATGGTGGGAACGCCT | 427 | 57 | FRAI | FRAI F | ACTTCAATCCGACAATGAATGGG | 546 | 56 | FRAI | FRAI R | ACAGAGCGAAATTCTATATC | 546 | 56 | FRAII | FRAII F | GGCGATACCGGTGGCAATTCTT | 689 | 55 | FRAII | FRAII R | CATGGGTATCCGTTGTCGTT | 689 | 55 |
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