Research Article

Mechanism of Gene Amplification via Yeast Autonomously Replicating Sequences

Table 3

Primers used for the PCR amplification of APS used in this study.

APSPrimer Primer sequence
(5′-3′)
Product size (bp) (°C)

ARS315315 FGGGATACGACCGGTCCTGAGAAA42856
ARS315315 RCCGCAAAGATGTGCGGGAAATTCT42856
ARS606606 FGGTCTTCTTGATAATTCTGTGGGCGCT36757
ARS606606 RGGTACCATATGAAACTCCAGCAGC36757
ARS13051305 FTGGCCATTGCGTTGTCATTGAGTG28159
ARS13051305 RGGGTGGAGTAATGGGTTTCACAAAGG28159
ARS14261426 FACCGTAGCCGTAACGTTTAATGCC32857
ARS14261426 RCGAGGAGATCCTCTTCTCTTTGCT32857
ARS15121512 FTCCAATGGCTTAGTAGCTGCCAGA38159
ARS15121512 RTGACAACAAGGAGGACAGCGAACA38159
PO5PO5 FGGGTGCGATCATACAGCGT 32049
PO5PO5 RGGGTGCCAACACTAGGACTTC32049
PL5PL5 FGAGTTCTGATGGGATCCGGTG35051
PL5PL5 RCGCTTGGGCTAGAGCAGTAC35051
Ste20-YF1YF1FAAATGTCCAAATATACACGATAAG61354
Ste20-YF1YF2RAGAACTACTATGGTGGGAACG61354
Ste20-YF2YF2FTGTGTTGTCAGCAGAATGATCCGGG52953
Ste20-YF2YF2RCGAGGTAGCAAGCAACCCAAACTT52953
Ste20-YF3YF3FATGAAGCAATTCCGTAGCGCTTGC53154
Ste20-YF3YF3RAGGCGACCTTTGGGTCATTATGGA53154
Ste20-YF4YF4FTGCTCGTTGTCGTTATCGTCGTCA42757
Ste20-YF4YF4RAGAACTACTATGGTGGGAACGCCT42757
FRAI FRAI FACTTCAATCCGACAATGAATGGG54656
FRAI FRAI RACAGAGCGAAATTCTATATC54656
FRAII FRAII FGGCGATACCGGTGGCAATTCTT68955
FRAII FRAII R CATGGGTATCCGTTGTCGTT68955