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The Scientific World Journal
Volume 2015 (2015), Article ID 595073, 8 pages
http://dx.doi.org/10.1155/2015/595073
Research Article

In Vitro Seeds Germination and Seedling Growth of Bambara Groundnut (Vigna subterranea (L.) Verdc. (Fabaceae))

1Laboratoire de Biologie et Amélioration des Productions Végétales, UFR des Sciences de la Nature, Université Nangui Abrogoua, 02 BP 801, Abidjan 02, Côte d’Ivoire
2Laboratoire de Physiologie Végétale, UFR Biosciences, Université Félix Houphouët-Boigny, 22 BP 582, Abidjan 22, Côte d’Ivoire

Received 10 August 2015; Accepted 20 September 2015

Academic Editor: Tariq Mahmood

Copyright © 2015 Mongomaké Koné et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Bambara groundnut (Vigna subterranea (L.) Verdc.) is an indigenous grain legume. It occupies a prominent place in the strategies to ensure food security in sub-Saharan Africa. Development of an efficient in vitro regeneration system, a prerequisite for genetic transformation application, requires the establishment of optimal conditions for seeds germination and plantlets development. Three types of seeds were inoculated on different basal media devoid of growth regulators. Various strengths of the medium of choice and the type and concentration of carbon source were also investigated. Responses to germination varied with the type of seed. Embryonic axis (EA) followed by seeds without coat (SWtC) germinated rapidly and expressed a high rate of germination. The growth performances of plantlets varied with the basal medium composition and the seeds type. The optimal growth performances of plants were displayed on half strength MS basal medium with SWtC and EA as source of seeds. Addition of 3% sucrose in the culture medium was more suitable for a maximum growth of plantlets derived from EA.