Qualitative and Quantitative Analysis of the Major Constituents in Chinese Medical Preparation Lianhua-Qingwen Capsule by UPLC-DAD-QTOF-MS

Table 1

Quantitative results of 12 compounds in LQC extracted by different methods.

Content (g/g)	Methods
	30%^a	60%	90%	60%	60%	60%	60%	60%	60%
	30 min^b	30 min	30 min	30 min	30 min	30 min	15 min	30 min	45 min
	1 : 100^c	1 : 100	1 : 100	1 : 50	1 : 100	1 : 200	1 : 100	1 : 100	1 : 100
Salidroside	1726.28	1701.25	1622.02	1522.31	1701.25	1656.32	1711.54	1701.25	1688.54
Chlorogenic acid	2444.97	2492.15	2216.89	2285.43	2492.15	2289.27	2492.62	2492.15	2552.17
Forsythoside E	1583.93	1620.78	451.91	1462.09	1620.78	1402.53	1627.01	1620.78	1579.63
Cryptochlorogenic acid	1862.98	1851.64	667.50	1703.05	1851.64	1771.79	1837.89	1851.64	1857.95
Amygdalin	1424.11	1455.39	1442.56	1268.93	1455.39	1298.11	1395.97	1455.39	1431.38
Sweroside	816.19	813.18	789.32	747.06	813.18	772.67	812.51	813.18	808.24
Hyperin	135.10	151.73	167.26	140.84	151.73	140.80	152.72	151.73	157.67
Rutin	122.00	121.17	115.22	106.11	121.17	116.67	117.09	121.17	116.62
Forsythoside A	2484.60	2536.34	2661.79	2285.76	2536.34	2396.35	2543.87	2536.34	2521.10
Phillyrin	1660.26	1521.45	1551.59	1410.19	1521.45	1390.12	1523.01	1521.45	1551.52
Rhein	803.13	1102.06	1370.11	937.63	1102.06	932.05	956.38	1102.06	1054.77
Glycyrrhizic acid	1530.49	1680.43	1594.37	1437.40	1680.43	1619.99	1674.81	1680.43	1665.42

Extracting solvent: 30%, 60%, and 90% methanol-water solution.
^bUltrasonic time: 15 min, 30 min, and 45 min.
^cExtraction solvent multiples: 1 : 50, 1 : 100, and 1 : 200 expressed 50, 100, and 200 times per gram of sample.