Qualitative and Quantitative Analysis of the Major Constituents in Chinese Medical Preparation Lianhua-Qingwen Capsule by UPLC-DAD-QTOF-MS

Table 3

Linear regression, LODs and LOQs, intraday and interday precisions, repeatability, stability, and recovery for 12 compounds.

Compound	Regression equation^a; ()		Linear range; (g/mL)	LOD^b; (μg/mL)	LOQ^c; (μg/mL)	Intraday (RSD, %); ()	Interday (RSD, %); ()	Repeatability ()		Stability; (RSD, %)	Recovery ( = 6)
								Mean (g/g)	RSD (%)		Original; (g)	Spiked; (μg)	Detected; (μg)	Recovery; (%)	RSD; (%)
Salidroside		0.9999	5.27–84.35	1.06	3.50	0.12	0.26		0.61	1.38	355.91	354.20	707.50	99.28	1.24
Chlorogenic acid		0.9998	6.85–109.56	1.71	5.69	0.77	2.12		0.51	2.54	494.74	493.85	959.90	94.19	2.42
Forsythoside E		0.9995	4.85–77.64	1.21	4.04	0.67	2.25		1.07	2.67	351.81	351.05	677.26	92.99	3.54
Cryptochlorogenic acid		0.9997	6.65–106.47	1.66	5.53	1.10	1.63		1.02	2.46	405.90	406.60	784.00	93.10	1.43
Amygdalin		0.9998	3.91–62.57	0.98	3.26	0.79	0.93		1.74	2.48	297.08	298.62	576.39	93.58	3.32
Sweroside		0.9999	2.00–31.96	0.25	0.80	0.11	0.33	798.06 ±; 2.93	0.37	0.48	159.61	159.51	325.43	103.95	0.41
Hyperin		0.9999	0.20–3.21	0.051	0.16	0.78	0.62	92.03 ±; 0.80	0.87	0.83	18.41	18.24	37.07	102.29	1.82
Rutin		0.9993	0.53–8.50	0.072	0.21	0.41	2.24		1.66	1.57	36.83	36.81	72.50	96.90	2.34
Forsythoside A		0.9999	4.21–67.34	0.30	1.05	0.20	2.92		0.74	1.95	497.00	495.95	997.33	100.89	1.49
Phillyrin		0.9999	2.86–45.71	0.71	2.36	0.79	1.63		0.64	2.76	315.56	316.72	623.99	100.25	1.60
Rhein		0.9996	3.47–55.49	0.87	2.88	0.40	0.28		2.39	0.97	136.38	136.27	273.48	100.61	1.61
Glycyrrhizic acid		0.9999	5.27–84.35	0.092	0.33	0.34	2.09		1.08	2.15	324.55	323.939	652.16	101.31	3.27

is the peak area; is the concentration of standard solutions.
^bLOD refers to the limits of detection, .
^cLOQ refers to the limits of quantity, .