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The Scientific World Journal
Volume 2015, Article ID 959138, 8 pages
http://dx.doi.org/10.1155/2015/959138
Research Article

Use of Probiotics to Control Aflatoxin Production in Peanut Grains

1Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Bloco J, 4º Andar, Sala 171, Avenida Antônio Carlos 6627, CP 486, Pampulha, 31270-901 Belo Horizonte, MG, Brazil
2Laboratório de Microbiologia Ambiental e Biotecnologia, Universidade Federal do Tocantins, ALC No. 14 Campus Universitário, Avenida NS 15 Bloco II Sala 05, 77.001-090 Palmas, TO, Brazil
3Fundação Ezequiel Dias, Laboratório de Micologia e Micotoxinas, Rua Conde Pereira Carneiro 80, 30510010 Belo Horizonte, MG, Brazil

Received 15 April 2015; Revised 11 June 2015; Accepted 16 June 2015

Academic Editor: Aldo Corsetti

Copyright © 2015 Juliana Fonseca Moreira da Silva et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Probiotic microorganisms (Saccharomyces cerevisiae var. boulardii, S. cerevisiae UFMG 905, and Lactobacillus delbrueckii UFV H2b20) were evaluated as biological control agents to reduce aflatoxin and spore production by Aspergillus parasiticus IMI 242695 in peanut. Suspensions containing the probiotics alone or in combinations were tested by sprinkling on the grains followed by incubation for seven days at 25°C. All probiotic microorganisms, in live and inactivated forms, significantly reduced A. parasiticus sporulation, but the best results were obtained with live cells. The presence of probiotics also altered the color of A. parasiticus colonies but not the spore morphology. Reduction in aflatoxin production of 72.8 and 65.8% was observed for S. boulardii and S. cerevisiae, respectively, when inoculated alone. When inoculated in pairs, all probiotic combinations reduced significantly aflatoxin production, and the best reduction was obtained with S. boulardii plus L. delbrueckii (96.1%) followed by S. boulardii plus S. cerevisiae and L. delbrueckii plus S. cerevisiae (71.1 and 66.7%, resp.). All probiotics remained viable in high numbers on the grains even after 300 days. The results of the present study suggest a different use of probiotics as an alternative treatment to prevent aflatoxin production in peanut grains.