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The Scientific World Journal
Volume 2016 (2016), Article ID 4305252, 7 pages
http://dx.doi.org/10.1155/2016/4305252
Research Article

De Novo Transcriptome Sequencing of Olea europaea L. to Identify Genes Involved in the Development of the Pollen Tube

1Consiglio per la Ricerca in Agricoltura e l’Analisi dell’Economia Agraria (CREA), Centro di Ricerca per l’Olivicoltura e l’Industria Olearia (OLI), 87036 Rende, Italy
2Università della Calabria, Dipartimento di Biologia, Ecologia e Scienze della Terra, Ponte Pietro Bucci, 87036 Arcavacata di Rende, Italy
3Dipartimento di Farmacia e Scienze della Salute e della Nutrizione, Università della Calabria, Polifunzionale, Arcavacata, 87036 Rende, Italy

Received 12 October 2015; Revised 17 January 2016; Accepted 20 January 2016

Academic Editor: Mali Salmon

Copyright © 2016 Domenico Iaria et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In olive (Olea europaea L.), the processes controlling self-incompatibility are still unclear and the molecular basis underlying this process are still not fully characterized. In order to determine compatibility relationships, using next-generation sequencing techniques and a de novo transcriptome assembly strategy, we show that pollen tubes from different olive plants, grown in vitro in a medium containing its own pistil and in combination pollen/pistil from self-sterile and self-fertile cultivars, have a distinct gene expression profile and many of the differentially expressed sequences between the samples fall within gene families involved in the development of the pollen tube, such as lipase, carboxylesterase, pectinesterase, pectin methylesterase, and callose synthase. Moreover, different genes involved in signal transduction, transcription, and growth are overrepresented. The analysis also allowed us to identify members in actin and actin depolymerization factor and fibrin gene family and member of the Ca2+ binding gene family related to the development and polarization of pollen apical tip. The whole transcriptomic analysis, through the identification of the differentially expressed transcripts set and an extended functional annotation analysis, will lead to a better understanding of the mechanisms of pollen germination and pollen tube growth in the olive.