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The Scientific World Journal
Volume 2017, Article ID 5260106, 6 pages
Research Article

Addition of Wollastonite Fibers to Calcium Phosphate Cement Increases Cell Viability and Stimulates Differentiation of Osteoblast-Like Cells

1Department of Structural and Functional Biology, Biology Institute, University of Campinas (UNICAMP), Campinas, SP, Brazil
2Department of Physiological Science, Biomaterials Laboratory, Pontifical Catholic University of Sao Paulo (PUC-SP), Sorocaba, SP, Brazil
3Bioceramics Laboratory, Science and Technology Institute, Federal University of São Paulo (UNIFESP), São José dos Campos, SP, Brazil
4Department of Physical Chemistry, Chemistry Institute, University of Campinas (UNICAMP), Campinas, SP, Brazil
5Department of Materials Engineering, Faculty of Mechanical Engineering, University of Campinas (UNICAMP), Campinas, SP, Brazil

Correspondence should be addressed to Juliana Almeida Domingues; moc.liamtoh@oib_dajadiemla

Received 21 March 2017; Revised 7 July 2017; Accepted 19 July 2017; Published 21 August 2017

Academic Editor: Silvia Scaglione

Copyright © 2017 Juliana Almeida Domingues et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Calcium phosphate cement (CPC) that is based on α-tricalcium phosphate (α-TCP) is considered desirable for bone tissue engineering because of its relatively rapid degradation properties. However, such cement is relatively weak, restricting its use to areas of low mechanical stress. Wollastonite fibers (WF) have been used to improve the mechanical strength of biomaterials. However, the biological properties of WF remain poorly understood. Here, we tested the response of osteoblast-like cells to being cultured on CPC reinforced with 5% of WF (CPC-WF). We found that both types of cement studied achieved an ion balance for calcium and phosphate after 3 days of immersion in culture medium and this allowed subsequent long-term cell culture. CPC-WF increased cell viability and stimulated cell differentiation, compared to nonreinforced CPC. We hypothesize that late silicon release by CPC-WF induces increased cell proliferation and differentiation. Based on our findings, we propose that CPC-WF is a promising material for bone tissue engineering applications.