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The Scientific World Journal
Volume 2017, Article ID 5873648, 7 pages
https://doi.org/10.1155/2017/5873648
Research Article

Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan

1Faculty of Pharmacy, University of Balochistan, Quetta, Pakistan
2Department of Health, Government of Balochistan, Quetta, Pakistan
3Balochistan University of Information Technology, Engineering & Management Sciences (BUITEMS), Quetta, Pakistan
4College of Pharmacy, University of Texas at Austin, Austin, TX, USA

Correspondence should be addressed to Rahman Gul; moc.oohay@inatod.lug

Received 22 January 2017; Revised 19 February 2017; Accepted 23 February 2017; Published 13 March 2017

Academic Editor: Valdir Cechinel Filho

Copyright © 2017 Rahman Gul et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of this study was to evaluate the antioxidant activity, screening the phytogenic chemical compounds, and to assess the alkaloids present in the E. intermedia to prove its uses in Pakistani folk medicines for the treatment of asthma and bronchitis. Antioxidant activity was analyzed by using 2,2-diphenyl-1-picryl-hydrazyl-hydrate assay. Standard methods were used for the identification of cardiac glycosides, phenolic compounds, flavonoids, anthraquinones, and alkaloids. High performance liquid chromatography (HPLC) was used for quantitative purpose of ephedrine alkaloids in E. intermedia. The quantitative separation was confirmed on Shimadzu 10AVP column (Shampack) of internal diameter (id) 3.0 mm and 50 mm in length. The extract of the solute in flow rate of 1 ml/min at the wavelength 210 nm and methanolic extract showed the antioxidant activity and powerful oxygen free radicals scavenging activities and the IC50 for the E. intermedia plant was near to the reference standard ascorbic acid. The HPLC method was useful for the quantitative purpose of ephedrine (E) and pseudoephedrine (PE) used for 45 samples of one species collected from central habitat in three districts (Ziarat, Shairani, and Kalat) of Balochistan. Results showed that average alkaloid substance in E. intermedia was as follows: PE (0.209%, 0.238%, and 0.22%) and E (0.0538%, 0.0666%, and 0.0514%).