Table of Contents Author Guidelines Submit a Manuscript
The Scientific World Journal
Volume 2017, Article ID 9064531, 9 pages
https://doi.org/10.1155/2017/9064531
Research Article

Wound Myiasis Caused by Sarcophaga (Liopygia) Argyrostoma (Robineau-Desvoidy) (Diptera: Sarcophagidae): Additional Evidences of the Morphological Identification Dilemma and Molecular Investigation

1Dipartimento di Scienze Agrarie, degli Alimenti e dell’Ambiente, Università degli Studi di Foggia, Via Napoli 25, Foggia, Italy
2Unità Operativa di Geriatria, Ospedale M. Bufalini, AUSL della Romagna, Ravenna, Italy
3Dipartimento di Sanità Pubblica, AUSL della Romagna, Ravenna, Italy
4Chair of Ecology and Biogeography, Faculty of Biology and Environmental Protection, Nicolaus Copernicus University, Lwowska 1, 87-100 Toruń, Poland

Correspondence should be addressed to Annunziata Giangaspero; ti.gfinu@orepsagnaig.ataiznunna

Received 13 October 2016; Accepted 8 December 2016; Published 29 January 2017

Academic Editor: Gabriella Cancrini

Copyright © 2017 Annunziata Giangaspero et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In Mediterranean countries, Sarcophaga (Liopygia) crassipalpis, Sarcophaga (L.) argyrostoma, and Sarcophaga (L.) cultellata share the same ecological niche and can be responsible of myiasis. In this study, the main morphological characters of a larva found in a hospitalized woman were described and illustrated by light and SEM microscopy and the features discussed. Then, a fragment within the mitochondrial encoded cytochrome c oxidase subunit I (coxI) gene of ~735 bp was amplified and sequenced. The molecular investigation was necessary to confirm the species Sarcophaga (Liopygia) argyrostoma (99% of identity). Our findings showed that morphological descriptions of larvae of three Mediterranean species of Liopygia available in several papers might not be clear enough to allow for comparison and correct identification. Until results of reliable comparative studies of larvae of all three species will be available, the use of molecular tools is crucial, to avoid misleading or incomplete identification, and in particular when a myiasis becomes a legal issue.