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The Scientific World Journal
Volume 2018, Article ID 6218430, 9 pages
https://doi.org/10.1155/2018/6218430
Research Article

Profiling of the Major Phenolic Compounds and Their Biosynthesis Genes in Sophora flavescens Aiton

1Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea
2Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Republic of Korea
3Korea Research Institute of Bioscience and Biotechnology, 181 Ipsin-gil, Jeongeup-si, Jeollabuk-do 56212, Republic of Korea
4Korea Research Institute of Bioscience and Biotechnology, Yeongudanji-ro 30, Ochang-eup, Cheongwon-gu, Cheongju-si 28116, Republic of Korea
5Systems and Bioengineering, University of Science and Technology, 217 Gajung-ro, Yuseong-gu, Daejeon 34113, Republic of Korea

Correspondence should be addressed to HyeRan Kim; rk.er.bbirk@rhmik

Received 31 October 2017; Accepted 18 January 2018; Published 1 March 2018

Academic Editor: Valdir Cechinel Filho

Copyright © 2018 Jeongyeo Lee et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Sophorae Radix (Sophora flavescens Aiton) has long been used in traditional medicine in East Asia due to the various biological activities of its secondary metabolites. Endogenous contents of phenolic compounds (phenolic acid, flavonol, and isoflavone) and the main bioactive compounds of Sophorae Radix were analyzed based on the qualitative HPLC analysis and evaluated in different organs and at different developmental stages. In total, 11 compounds were detected, and the composition of the roots and aerial parts (leaves, stems, and flowers) was significantly different. trans-Cinnamic acid and p-coumaric acid were observed only in the aerial parts. Large amounts of rutin and maackiain were detected in the roots. Four phenolic acid compounds (benzoic acid, caffeic acid, ferulic acid, and chlorogenic acid) and four flavonol compounds (kaempferol, catechin hydrate, epicatechin, and rutin) were higher in aerial parts than in roots. To identify putative genes involved in phenolic compounds biosynthesis, a total of 41 transcripts were investigated. Expression patterns of these selected genes, as well as the multiple isoforms for the genes, varied by organ and developmental stage, implying that they are involved in the biosynthesis of various phenolic compounds both spatially and temporally.