Research Article | Open Access
Effects of the Photosystem II Inhibitors CCCP and DCMU on Hydrogen Production by the Unicellular Halotolerant Cyanobacterium Aphanothece halophytica
The unicellular halotolerant cyanobacterium Aphanothece halophytica is a potential dark fermentative producer of molecular hydrogen (H2) that produces very little H2 under illumination. One factor limiting the H2 photoproduction of this cyanobacterium is an inhibition of bidirectional hydrogenase activity by oxygen (O2) obtained from splitting water molecules via photosystem II activity. The present study aimed to investigate the effects of the photosystem II inhibitors carbonyl cyanide m-chlorophenyl hydrazone (CCCP) and 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) on H2 production of A. halophytica under light and dark conditions and on photosynthetic and respiratory activities. The results showed that A. halophytica treated with CCCP and DCMU produced H2 at three to five times the rate of untreated cells, when exposed to light. The highest H2 photoproduction rates, and μmol H2 g−1 dry weight h−1, were found in cells treated with 0.5 μM CCCP and 50 μM DCMU, respectively. Without inhibitor treatment, A. halophytica incubated in the dark showed a significant increase in H2 production compared with cells that were incubated in the light. Only CCCP treatment increased H2 production of A. halophytica during dark incubation, because CCCP functions as an uncoupling agent of oxidative phosphorylation. The highest dark fermentative H2 production rate of μmol H2 g−1 dry weight h−1 was found in cells treated with 0.5 μM CCCP after 2 h of dark incubation. Under illumination, CCCP and DCMU inhibited chlorophyll fluorescence, resulting in a low level of O2, which promoted bidirectional hydrogenase activity in A. halophytica cells. In addition, only CCCP enhanced the respiration rate, further reducing the O2 level. In contrast, DCMU reduced the respiration rate in A. halophytica.
Molecular hydrogen (H2) has attracted a great deal of interest from researchers because H2 combustion liberates a high heating value with 141.6 MJ kg−1  and does not emit polluting gases to the environment. H2 production is a result of many processes, including physical, chemical, and biological processes. Biological H2 production can be established in many kinds of microorganisms such as photosynthetic bacteria, fermentative bacteria, green algae, and cyanobacteria . Among these microorganisms, cyanobacteria show high capability because they can generate H2 using electrons obtained from a light reaction of the photosynthetic pathway and/or from the degradation of storage carbohydrates within cells in darkness [3, 4].
The unicellular cyanobacterium Aphanothece halophytica is a halotolerant microorganism that can grow in a wide range of salinity from 0.25 to 3.0 M NaCl . A. halophytica produces a large amount of dark fermentative H2 compared with other marine cyanobacteria [6, 7]. H2 production by A. halophytica is catalyzed by bidirectional hydrogenase and occurs particularly under nitrogen-deprived and dark anaerobic conditions [6–8]. Hydrogenase is the only enzyme that catalyzes both H2 uptake and H2 production in this organism . Due to the high sensitivity of bidirectional hydrogenase to oxygen (O2) , which is the main product when photosystem II (PSII) activity splits a water molecule, H2 production by A. halophytica decreases in the light . To enhance H2 production by A. halophytica, O2 must be removed. One way to eliminate the generation of O2 from splitting water molecules during photolysis is to use photosystem II inhibitors.
Carbonyl cyanide m-chlorophenyl hydrazone (CCCP) has long been recognized as a photosystem II inhibitor of cyanobacteria and green algae . CCCP has been shown to inhibit the photochemical activity of PSII under illumination in cyanobacteria Synechocystis sp. PCC 6803 , Synechococcus sp. PCC 7942 , Nostoc sp., and Lyngbya sp.  and green algae Chlorella ellipsoidea  and Platymonas subcordiformis . This inhibition leads to a decrease in O2 production. CCCP can also function as an uncoupling agent of oxidative phosphorylation . It disrupts the proton motive force by releasing protons across the thylakoid membrane, resulting in an inhibition of ATP synthesis. Consequently, a large number of electrons and protons can be transferred to bidirectional hydrogenase to enhance H2 production . It has been reported that CCCP increased H2 production in the cyanobacteria Oscillatoria chalybea and Synechocystis sp. PCC 6803  and in the green algae Chlamydomonas reinhardtii , P. subcordiformis [17, 20, 21], and Platymonas helgolandica var. tsingtaoensis . In addition, this inhibition of ATP synthesis resulted in an increase in the rate of dark respiration in cyanobacteria Anabaena variabilis  and Anacystis nidulans  and green alga C. reinhardtii .
Another PSII inhibitor is 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) . DCMU can block electron transfer between the primary quinone electron accepter () and secondary quinone electron accepter () on the reducing side of PSII . This interrupts the photosynthetic electron transport chain in photosynthesis and thus reduces the generation of O2 from splitting water molecules via PSII. DCMU has been shown to inhibit PSII activity in cyanobacteria Aphanocapsa 6308 , Nostoc sp., and Lyngbya sp.  and green alga Scenedesmus quadricauda . DCMU influences other cellular processes, such as cyclic phosphorylation, chlorophyll synthesis, and fatty acid synthesis . In previous reports, H2 production of the cyanobacteria Anabaena spp. strains CA and 1F , Anabaena cylindrica , Anabaena 7120 , and the green alga P. helgolandica var. tsingtaoensis  was increased in the presence of DCMU under illumination. In addition, DCMU caused the inhibition of dark respiration in cyanobacteria Plectonema boryanum  and Anabaena flos-aquae .
The goal of the present study was to investigate the effects of the PSII inhibitors CCCP and DCMU on H2 production by the cyanobacterium A. halophytica. The data will improve our understanding of the functional relationships between H2 metabolism and photosynthetic and respiration efficiency. The knowledge gained in this study will be useful to enhance H2 production by A. halophytica under light or dark conditions by a use of the effective PSII inhibitors, DCMU and CCCP. H2 evolution by this cyanobacterium might be one of the most promising ways to produce alternative clean energy fuel in the future.
2. Materials and Methods
2.1. Growth Conditions
A. halophytica was cultivated in a 250-mL Erlenmeyer flask containing 100 mL of BG11 medium (pH 7.4)  supplemented with Turk Island salt solution . The initial cell concentration was adjusted to an optical density of approximately 0.1 at 730 nm. Cells were shaken at 120 rpm at 30°C under a cool white light intensity of 30 μmol photons m−2 s−1 for 7 days.
2.2. Application of CCCP and DCMU
After the 7 days of growth, 100 mL of A. halophytica cells was harvested by centrifugation at 8,000 x g at 4°C for 10 min. The cell pellet was washed twice and resuspended in 100 mL of nitrogen-deprived BG11 (BG110) supplemented with Turk Island salt solution. The cells in suspension were transferred to a 250-mL Erlenmeyer flask and incubated on a rotary shaker at 120 rpm at 30°C under 30 μmol photons m−2 s−1 for 24 h. Cells were subsequently harvested by centrifugation and resuspended in 5 mL of BG110 supplemented with Turk Island salt solution. Next, a 5-mL volume of the cells in suspension was transferred to a 10-mL glass vial. CCCP and DCMU were subsequently added into the cell suspension with final concentrations of 0–5 μM and 0–250 μM, respectively. The vials were sealed with a rubber stopper with an aluminum rim and incubated at 30°C under 30 μmol photons m−2 s−1 for 2 h. The vials were subsequently purged with argon gas for 10 min to establish anaerobic conditions. The vials were further incubated under light at 30°C. Aliquots of cells in suspension after incubation for 2, 24, 48, 72, and 96 h were collected for analysis of cell and chlorophyll concentrations. Bidirectional hydrogenase activity, photosynthetic efficiency of PSII by chlorophyll fluorescence measurement, and dark respiration rate were analyzed after the cells were exposed to the light for 2 h. H2 production was analyzed after the cells were placed in both light and dark conditions for 2 h.
2.3. Measurement of Cell Concentration and Chlorophyll-a Concentration
The cell concentration of A. halophytica was analyzed using a hemocytometer under a microscope (Nikon Eclipse Ci-L, Japan). To analyze the chlorophyll-a concentration, 1 mL of a cell culture was harvested by centrifugation at 8,000 x g at 4°C for 10 min. A 1-mL volume of 90% (v/v) methanol was added to the cell pellet and mixed by vortexing. The mixture was incubated at 25°C in the dark for 1 h. The chlorophyll-a content was determined by measuring the absorbance of the extract at 665 nm by spectrophotometer .
2.4. Measurement of H2 Production
H2 concentration in 500 μL of headspace gas was analyzed by gas chromatograph (Hewlett-Packard HP5890A, Japan) with a molecular sieve 5°A, 60/80 mesh packed column using a thermal conductivity detector under previously described conditions . The H2 production rate was calculated as a term of μmol H2 g−1 dry weight h−1.
2.5. Measurement of Bidirectional Hydrogenase Activity
The bidirectional hydrogenase activity in the A. halophytica sample was determined after cells were incubated with various concentrations of CCCP and DCMU under the light for 2 h. Bidirectional hydrogenase activity was measured in the presence of dithionite-reduced methyl viologen. The assay contained 1 mL of cells in suspension and 1 mL of 25 mM phosphate buffer (pH 7.0) containing 2.5 mM methyl viologen and 10 mM sodium dithionite . The reaction mixture was incubated under dark anaerobic conditions at 25°C for 15 min before H2 production was measured using gas chromatography, under previously described conditions . Bidirectional hydrogenase activity was calculated in terms of μmol H2 g−1 dry weight min−1.
2.6. Dark Respiration Rate Measurement
The dark respiration rate was monitored at 25°C using a Clark-type oxygen electrode (Hansatech, UK). First, 2 mL of cells in suspension was added to the chamber and illuminated under 300 μmol photons m−2 s−1 of white light until the O2 concentration was constant. Then, the respiratory rate was measured as O2 consumption in the dark for 15 min. The dark respiration rate was calculated as a term of μmol O2 g−1 dry weight min−1.
2.7. Fluorescence Emission Spectra Measurement
Chlorophyll fluorescence emission spectra were determined at room temperature by spectrofluorometer (Jasco, Model FP-6300, Japan). First, 1 mL of cyanobacteria treated and not treated with CCCP and DCMU was exposed to light at 2,000 μmol photons m−2 s−1 at room temperature for 10 min prior to chlorophyll fluorescence measurement, following Joshua et al. . The chlorophyll fluorescence measurement was carried out using the excitation wavelength at 437 nm.
2.8. Statistical Data Analysis
The data in this study were statistically compared using a one-way ANOVA with Duncan’s post hoc test. Differences between means were considered significant at 0.05 (p < 0.05). Data were analyzed using IBM SPSS statistic 23 (IBM Corp., USA).
3.1. Effects of CCCP and DCMU on Cell and Chlorophyll-a Concentrations under N-Deprivation
After A. halophytica cells were incubated in a nitrogen-deprived medium containing various concentrations of CCCP (0–5 μM) and DCMU (0–250 μM) under light for 2, 24, 48, 72, and 96 h, cell and chlorophyll-a concentrations were measured. The concentrations of both decreased after the cells were incubated in BG110 containing CCCP or DCMU. Both concentrations slightly decreased in the first 2 h of incubation and continued to decrease to 96 h of incubation (Figure 1). Higher concentrations and longer incubation times of CCCP and DCMU led to an obvious decrease in the cell and chlorophyll-a concentrations of A. halophytica (Figure 1).
3.2. Effects of CCCP and DCMU on H2 Production
A. halophytica cells were treated with various concentrations of CCCP (0–5 μM) and DCMU (0–250 μM) and incubated under light at 30°C for 2 h before H2 was measured under dark and light anaerobic conditions. The cells that were incubated in the dark, with or without CCCP and DCMU treatment, generated H2 at a higher rate than did those incubated under the light (Figure 2). For cells treated with CCCP, the H2 production rates, under conditions of both illumination and darkness, were significantly increased, corresponding with the higher concentrations of CCCP (Figures 2(a) and 2(b)). However, the highest concentration of CCCP (5 μM) resulted in the lowest H2 production rates (Figures 2(a) and 2(b)). The highest H2 production rates of and μmol H2 g−1 dry weight h−1 were found in A. halophytica cells treated with 0.5 μM CCCP under light and dark conditions (Figures 2(a) and 2(b)). These H2 production rates were approximately threefold higher than those of cells without CCCP treatment.
In the presence of DCMU, A. halophytica showed a higher H2 production rate only when cells were incubated under light (Figure 2(c)). Dark fermentative H2 production was not increased in cells treated with all concentrations of DCMU (Figure 2(d)). Interestingly, in the presence of 250 μM DCMU, dark fermentative H2 production was obviously decreased and was lower than that in cells without DCMU treatment (Figure 2(d)). The highest H2 production rates of and μmol H2 g−1 dry weight h−1 were found in A. halophytica cells treated with 50 μM DCMU under light and dark conditions (Figures 2(c) and 2(d)). The results indicated that CCCP increased the H2 production rate under light and dark conditions and that DCMU increased it only under the light.
3.3. Effects of CCCP and DCMU on Bidirectional Hydrogenase Activity
To determine whether an increase in H2 production after CCCP and DCMU treatment resulted from increased bidirectional hydrogenase activity, A. halophytica cells were treated or not treated with CCCP or DCMU and incubated under the light for 2 h before bidirectional hydrogenase activity was measured. The results showed that bidirectional hydrogenase activity was higher when cells were treated with higher CCCP and DCMU concentrations. The highest bidirectional hydrogenase activity levels of and μmol H2 g−1 dry weight min−1 were found in cells treated with 0.5 μM CCCP and 50 μM DCMU, respectively (Figures 3(a) and 3(b)). When CCCP and DCMU concentrations exceeded these concentrations, the bidirectional hydrogenase activity level decreased (Figures 3(a) and 3(b)). As expected, bidirectional hydrogenase activities were related to H2 production rates (Figures 2 and 3).
3.4. Effect of CCCP and DCMU on Chlorophyll Fluorescence
Chlorophyll fluorescence emission spectra of A. halophytica cells treated with various concentrations of CCCP and DCMU under the light for 2 h were measured. The results showed that the chlorophyll fluorescence emission spectra of A. halophytica cells treated with higher concentrations of CCCP or DCMU were significantly lower than those that were not treated (Figure 4), suggesting that these inhibitors could inhibit PSII efficiency, leading to a decrease in the O2 level in vials (data not shown), an increase in bidirectional hydrogenase activity (Figure 3), and an increase in H2 production rate (Figure 2).
3.5. Effects of CCCP and DCMU on Dark Respiration
The measurement of dark respiration rate was performed in A. halophytica cells after they were treated or not treated with CCCP or DCMU. Cells treated with 0.01–1 μM CCCP showed higher dark respiration rates than those that were not treated with CCCP (Figure 5(a)). The highest dark respiration rate of μmol O2 g−1 dry weight min−1 was found in cells that were treated with 0.5 μM CCCP, and the lowest dark respiration rate was found in cells treated with 5 μM CCCP (Figure 5(a)). DCMU concentrations higher than 0.5 μM reduced the dark respiration rate of A. halophytica cells (Figure 5(b)).
4.1. Effects of CCCP and DCMU on Cell Inhibition
The treatment of A. halophytica cells with CCCP led to a reduction in cell concentration (Figure 1(a)) and chlorophyll-a concentration (Figure 1(b)), especially in cells that received high concentrations of CCCP over long-term incubations (Figures 1(a) and 1(b)). In the absence of CCCP, cells did not show any changes in the cell and chlorophyll-a concentrations. The cell and chlorophyll-a concentrations also did not increase because all cells were incubated in BG110 lacking in NaNO3, which is a nitrogen source for cyanobacterial growth. CCCP, which functions as the PSII inhibitor, inhibited the rate of electron flow through the photosynthetic electron transport chain in the thylakoid membrane of cyanobacterial cells . Consequently, cell and chlorophyll concentrations in A. halophytica were decreased. These results were consistent with previous studies showing a decrease in the optical density and cell concentration of other microalgae [39, 40]. In Synechococcus sp., the optical density at 750 nm and viable cell count were decreased after the addition of 10 μM CCCP . The unicellular green alga C. reinhardtii showed MICs at 8.5 and 14.6 μM for CCCP under heterotrophic and photoautotrophic growth conditions, respectively . In case of chlorophyll content, the green algae P. helgolandica var. tsingtaoensis and C. reinhardtii showed a decrease in chlorophyll content when cell cultures were treated with 15 μM CCCP [19, 22].
The treatment of A. halophytica with DCMU also caused a reduction in cell concentration (Figure 1(c)) and chlorophyll-a concentration (Figure 1(d)). DCMU, similar to CCCP, is responsible for inhibition of photosynthetic activity; therefore, DCMU treatment inhibited cell growth and chlorophyll concentrations as shown in Figures 1(c) and 1(d). These results agreed with previous studies. In Synechocystis sp. PCC 6803, the optical density at 730 nm was decreased in the presence of 0.1 μM DCMU . DCMU at 0.1 μM caused a significant reduction in the growth of colonies of algae Eudorina elegans  and Nannochloropsis . In the presence of DCMU, the chlorophyll concentrations of the N2-fixing cyanobacteria Nostoc sp. G3 and A. variabilis obviously decreased [44, 45]. The results of the present study suggested that high concentrations of CCCP and DCMU and long-term incubation caused cell toxicity and death.
4.2. H2 Production of A. halophytica under Light and Dark Conditions
H2 production rates by A. halophytica cells incubated in the dark were higher than those incubated under the light, in the presence or absence of inhibitors (Figure 2), indicating that light can inhibit H2 production by A. halophytica. A. halophytica cells under the light produced more O2 than did those in the dark (data not shown) due to the generation of O2 from the splitting of water molecules via PSII activity in the thylakoid membrane. O2 inhibits the bidirectional hydrogenase activity of A. halophytica cells, resulting in lower H2 production. In the dark, A. halophytica cells had reduced photolysis but engaged in dark respiration, leading to a lower O2 concentration in the system and enhanced H2 production. Moreover, nitrogen-deprived cells of A. halophytica were able to generate more H2 from electrons acquired through the degradation of stored glycogen under dark, anaerobic conditions than from photosynthesis under light conditions [6–8].
4.3. Effects of CCCP on H2 Production, Bidirectional Hydrogenase Activity, Photosynthetic Activity, and Dark Respiration
The CCCP-treated A. halophytica showed significantly higher H2 production under both light and dark conditions than CCCP-untreated cells. The highest H2 production rates of and μmol H2 g−1 dry weight h−1 were found in cultures treated with 0.5 μM CCCP and incubated under light and dark conditions, respectively (Figures 2(a) and 2(b)). These H2 production rates were approximately threefold higher than those in the absence of CCCP. CCCP-treated cells also produced less O2, as measured in the gas (data not shown). The lower O2 concentration in the glass vial caused increased bidirectional hydrogenase activity, as shown in Figure 3(a), and increased the H2 production rate (Figure 2). Our results agree with previous studies showing that H2 production rates of the cyanobacteria O. chalybea and Synechocystis sp. PCC 6803 treated with CCCP were higher than those of untreated cells . In green algae, H2 production by C. reinhardtii, P. subcordiformis, and Tetraselmis subcordiformis was also increased in CCCP-treated cells [17, 19, 46].
CCCP-treated A. halophytica cells showed an increase in the H2 production rate under dark conditions (Figure 2(b)). This enhancement most likely was not due to decreased PSII activity by CCCP, but to the inhibition of oxidative phosphorylation by another effect of CCCP as an uncoupler agent . In green algae, CCCP inhibits the flow of electrons in the electron transport chain and promotes the pumping of protons in the oxidative phosphorylation reaction by transporting protons across the thylakoid membrane [20, 47]. As a result, the activity of ATP synthase is reduced, and ATP synthesis is inhibited. The released or excess protons and electrons could be reduced by bidirectional hydrogenase to generate H2 .
To confirm the effects of CCCP on H2 production by A. halophytica, bidirectional hydrogenase activity, photosynthetic activity, and dark respiration rate were measured. A treatment of 0.5 μM CCCP produced the highest bidirectional hydrogenase activity level (Figure 3(a)), indicating that concentration of CCCP at 0.5 μM is optimal for promoting bidirectional hydrogenase activity in A. halophytica. In a previous study, a treatment with 10 μM CCCP could increase bidirectional hydrogenase activity in Anabaena siamensis TISTR 8012 . Therefore, the CCCP concentration influencing hydrogenase activity is species-dependent. However, the chlorophyll fluorescence intensity of A. halophytica cells decreased as CCCP concentrations increased (Figure 4(a)). Evidently, CCCP inhibited photosystem II activity, contributing to the lower chlorophyll fluorescence, as shown in Figure 4(a). Moreover, CCCP could inhibit ATP synthesis from working as an uncoupler of oxidative phosphorylation and subsequently increase the respiration rate, as shown in Figure 5(a). The decrease in O2 photoevolution, together with the increase in O2 consumption, promoted a low level of O2 in the system, which is favorable for bidirectional hydrogenase activity. Our results were similar to previous results reported in many cyanobacterial and green algal strains, demonstrating that CCCP reduced PSII photochemical activity [17, 19, 21, 46, 49] and enhanced the rate of dark respiration . It has been reported that the rate of dark respiration was markedly enhanced by addition of 5 and 10 μM CCCP to the cultures of A. variabilis  and A. nidulans . In C. reinhardtii, CCCP at 2.5 μM increased the dark respiration rate by 40% without influencing photosynthesis . However, in this study the effect of CCCP on the dark respiration rate in A. halophytica was dependent on the CCCP concentration. These data on the stimulation of H2 photoevolution and dark fermentative H2 production by CCCP treatment may be used to optimize H2 production by A. halophytica in the future.
4.4. Effects of DCMU on H2 Production, Bidirectional Hydrogenase Activity, Dark Respiration, and Photosynthetic Activity
DCMU-treated A. halophytica produced H2 at a significantly higher rate than did DCMU-untreated cells under light conditions (Figure 2(c)) but not under darkness (Figure 2(d)). Evidently, DCMU functioned as a PSII inhibitor in the light, leading to the reduction of O2 photoevolution from photolysis. Therefore, the decreased O2 level caused an increase of H2 production. These results were consistent with the previous results described for CCCP-treated cells. However, under dark conditions, the cyanobacterial cells could not perform photosynthesis and thus were unable to generate O2. Therefore, DCMU might not inactivate PSII activity under darkness, resulting in a constant H2 production rate compared with the untreated cells. In addition, it is likely that DCMU could not promote dark fermentative H2 production by A. halophytica. These results contrasted with those of studies in Synechocystis sp. PCC 6803, which reported higher H2 production in the presence of 75 μM DCMU under dark and anaerobic conditions [50, 51].
Under light conditions, the H2 production rate of 50 μM DCMU-treated A. halophytica cells was threefold higher than that of untreated cells (Figure 2(c)). This high rate resulted from the highest observed bidirectional hydrogenase activity in the present study, recorded in 50 μM DCMU-treated cells (Figure 3(b)). This result was consistent with the previous study showing that the highest bidirectional hydrogenase activity of A. siamensis TISTR 8012 was obtained when treating cells with 50 μM DCMU under nitrogen deprivation . In this study, it could be explained that the increased hydrogenase activity resulted from a decrease in the chlorophyll fluorescence intensity (Figure 4(b)) and/or the dark respiration rate (Figure 5(b)), indicating that DCMU caused the inhibition of both dark respiration and PSII activity. Our findings that H2 production increased after treatment with DCMU agreed with previous studies on cyanobacteria and green algae. In the cyanobacterium A. cylindrica, H2 production was improved in cells incubated with DCMU, due to the low level of O2 . H2 photoevolution also increased in cells of a new marine green alga, P. helgolandica var. tsingtaoensis which were treated with DCMU, as PSII photochemical activity during illumination was completely inhibited by DCMU . The similar result of DCMU inhibition on the photosynthetic electron transport system was reported in the cyanobacteria Aphanocapsa 6308 , A. nidulans , and A. siamensis TISTR 8012 . In contrast to CCCP result, DCMU treatment did not show an enhanced rate of respiration in A. halophytica but showed a significant decrease in respiration rate, especially with high DCMU concentrations (Figure 5(b)), suggesting that DCMU and CCCP possess different functions involved in the respiratory mechanism. Similar results were also found in A. flos-aquae  and Chlorella sp.  showing an inhibition of DCMU in respiration rates in the dark.
In the present study, high concentration of CCCP (5 μM) and DCMU (250 μM) induced a significant decrease of H2 production (Figure 2) due to the toxicity of CCCP and DCMU to A. halophytica cells. These results were confirmed by other experiments showing that too high concentrations of CCCP and DCMU reduced cell and chlorophyll concentrations (Figure 1), the bidirectional hydrogenase activity level (Figure 3), chlorophyll fluorescence intensities (Figure 4), and dark respiration rates (Figure 5).
Previous studies reported that, due to the limitation of O2 on bidirectional hydrogenase activity in the cyanobacterium A. halophytica, a very low level of H2 was detected after cells were exposed to illumination. In the present study, the well-known photosystem II inhibitors CCCP and DCMU were added to A. halophytica samples in an effort to enhance H2 production. Both CCCP and DCMU enhanced H2 production of A. halophytica under light conditions, whereas only CCCP enhanced H2 production under darkness. CCCP and DCMU functioned as PSII inhibitors during illumination, resulting in a decrease of chlorophyll fluorescence and O2 production in a glass vial. As a result, bidirectional hydrogenase activity was increased and H2 production was increased. In addition, CCCP functioned as an uncoupling agent of oxidative phosphorylation, decreasing both proton pumping and ATP synthesis, which resulted in an increase in the respiration rate. This effect helped increase H2 production after CCCP treatment under darkness. Our data showed that CCCP can increase H2 production by A. halophytica under both light and dark conditions. However, high concentration and long-term incubation of CCCP led to high cell toxicity. Since A. halophytica can grow in natural seawater supplemented with 1.76 mM NaNO3 , it would be useful if this cyanobacterium grown in natural seawater will produce long-term of H2 photohydrogen by using PSII inhibitors. This study needs further investigation.
The data used to support the findings of this study are included within the article.
Conflicts of Interest
The authors declare that there are no conflicts of interest regarding the publication of this paper.
This work was supported by the Faculty of Science, King Mongkut’s Institute of Technology Ladkrabang (KMITL) (grant number 2562-01-05-34). We would also like to acknowledge Associate Prof. Dr. Pattareeya Damrongsak and Mr. Ekkachai Rammarat from the Department of Physics, Faculty of Science, King Mongkut’s Institute of Technology Ladkrabang (KMITL), for their excellent assistance in chlorophyll fluorescence emission measurement. We would like to thank Editage (www.editage.com) for English language editing.
- J. H. Perry, Chemical Engineers Handbook, McGraw-Hill, New York, NY, USA, 1963.
- D. Das and T. N. Veziroǧlu, “Hydrogen production by biological processes: a survey of literature,” International Journal of Hydrogen Energy, vol. 26, no. 1, pp. 13–28, 2001.
- R. C. Prince and H. S. Kheshgi, “The photobiological production of hydrogen: Potential efficiency and effectiveness as a renewable fuel,” Critical Reviews in Microbiology, vol. 31, no. 1, pp. 19–31, 2005.
- P. Tamagnini, E. Leitão, P. Oliveira et al., “Cyanobacterial hydrogenases: diversity, regulation and applications,” FEMS Microbiology Reviews, vol. 31, no. 6, pp. 692–720, 2007.
- T. Takabe, A. Incharoensakdi, K. Arakawa, and S. Yokota, “CO2 fixation rate and RuBisCO content increase in the halotolerant cyanobacterium, Aphanothece halophytica, grown in high salinities,” Plant Physiology, vol. 88, no. 4, pp. 1120–1124, 1988.
- S. Taikhao, S. Junyapoon, A. Incharoensakdi, and S. Phunpruch, “Factors affecting biohydrogen production by unicellular halotolerant cyanobacterium Aphanothece halophytica,” Journal of Applied Phycology, vol. 25, no. 2, pp. 575–585, 2013.
- S. Taikhao, A. Incharoensakdi, and S. Phunpruch, “Dark fermentative hydrogen production by the unicellular halotolerant cyanobacterium Aphanothece halophytica grown in seawater,” Journal of Applied Phycology, vol. 27, no. 1, pp. 187–196, 2015.
- S. Phunpruch, S. Taikhao, and A. Incharoensakdi, “Identification of bidirectional hydrogenase genes and their co-transcription in unicellular halotolerant cyanobacterium Aphanothece halophytica,” Journal of Applied Phycology, vol. 28, no. 2, pp. 967–978, 2016.
- J. P. Houchins, “The physiology and biochemistry of hydrogen metabolism in cyanobacteria,” Biochimica et Biophysica Acta (BBA) - Reviews on Bioenergetics, vol. 768, no. 3-4, pp. 227–255, 1984.
- V. D. Samuilov, E. L. Barsky, O. N. Gubanova, V. V. Klimov, and Y. N. Kozlov, “Photoreduction of silicomolybdate in chloroplasts by agents accelerating the deactivation reactions of the water-oxidizing system,” FEBS Letters, vol. 357, no. 1, pp. 55–57, 1995.
- P. C. Meunier, R. L. Burnap, and L. A. Sherman, “Interaction of the photosynthetic and respiratory electron transport chains producing slow O2 signals under flashing light in Synechocystissp. PCC 6803,” Photosynthesis Research, vol. 45, no. 1, pp. 31–40, 1995.
- M. Torimura, A. Miki, A. Wadano, K. Kano, and T. Ikeda, “Electrochemical investigation of cyanobacteria Synechococcus sp. PCC7942-catalyzed photoreduction of exogenous quinones and photoelectrochemical oxidation of water,” Journal of Electroanalytical Chemistry, vol. 496, no. 1-2, pp. 21–28, 2001.
- J. M. Pisciotta, Y. Zou, and I. V. Baskakov, “Light-dependent electrogenic activity of cyanobacteria,” PLoS ONE, vol. 5, no. 5, Article ID e10821, pp. 1–10, 2010.
- Y. Matsuda and B. Colman, “Characterization of sulfate transport in the green alga Chlorella ellipsoidea,” Plant & Cell Physiology (PCP), vol. 36, no. 7, pp. 1291–1296, 1995.
- C.-F. Ji, J. Legrand, J. Pruvost, Z.-A. Chen, and W. Zhang, “Characterization of hydrogen production by Platymonas subcordiformis in torus photobioreactor,” International Journal of Hydrogen Energy, vol. 35, no. 13, pp. 7200–7205, 2010.
- P. G. Heytler, “Uncouplers of oxidative phosphorylation,” Methods in Enzymology, vol. 55, pp. 442–462, 1979.
- C. Ran, X. Yu, M. Jin, and W. Zhang, “Role of carbonyl cyanide m-chlorophenylhydrazone in enhancing photobiological hydrogen production by marine green alga Platymonas subcordiformis,” Biotechnology Progress, vol. 22, no. 2, pp. 438–443, 2006.
- R. Abdel-Basset and K. P. Bader, “Physiological analyses of the hydrogen gas exchange in cyanobacteria,” Journal of Photochemistry and Photobiology B: Biology, vol. 43, no. 2, pp. 146–151, 1998.
- D. Yang, Y. Zhang, D. K. Barupal et al., “Metabolomics of photobiological hydrogen production induced by CCCP in Chlamydomonas reinhardtii,” International Journal of Hydrogen Energy, vol. 39, no. 1, pp. 150–158, 2014.
- Y. Guan, W. Zhang, M. Deng, M. Jin, and X. Yu, “Significant enhancement of photobiological H2 evolution by carbonylcyanide m-chlorophenylhydrazone in the marine green alga Platymonas subcordiformis,” Biotechnology Letters, vol. 26, no. 13, pp. 1031–1035, 2004.
- Z. Guo, Z. Chen, W. Zhang, X. Yu, and M. Jin, “Improved hydrogen photoproduction regulated by carbonylcyanide m-chlorophenylhrazone from marine green alga Platymonas subcordiformis grown in CO2-supplemented air bubble column bioreactor,” Biotechnology Letters, vol. 30, no. 5, pp. 877–883, 2008.
- Y. Zhang, X. Fan, Z. Yang, H. Wang, D. Yang, and R. Guo, “Characterization of H2 photoproduction by a new marine green alga, Platymonas helgolandica var. tsingtaoensis,” Applied Energy, vol. 92, pp. 38–43, 2012.
- H. Imafuku and T. Katoh, “Intracellular ATP level and light-induced inhibition of respiration in a blue-green alga, anabaena variabilis,” Plant & Cell Physiology (PCP), vol. 17, no. 3, pp. 515–524, 1976.
- R. Shyam, A. S. Raghavendra, and P. V. Sane, “Role of dark respiration in photoinhibition of photosynthesis and its reactivation in the cyanobacterium Anacystis nidulans,” Physiologia Plantarum, vol. 88, no. 3, pp. 446–452, 1993.
- K. K. Singh, R. Shyam, and P. V. Sane, “Reactivation of photosynthesis in the photoinhibited green alga Chlamydomonas reinhardtii: role of dark respiration and of light,” Photosynthesis Research, vol. 49, no. 1, pp. 11–20, 1996.
- J. G. Metz, H. B. Pakrasi, M. Seibert, and C. J. Arntzer, “Evidence for a dual function of the herbicide-binding D1 protein in photosystem II,” FEBS Letters, vol. 205, no. 2, pp. 269–274, 1986.
- M. M. Allen, A. C. Turnburke, E. A. Lagace, and K. E. Steinback, “Effects of photosystem II herbicides on the photosynthetic membranes of the cyanobacterium Aphanocapsa 6308,” Plant Physiology, vol. 71, no. 2, pp. 388–392, 1983.
- J. Komenda, “Photosystem 2 photoinactivation and repair in the Scenedesmuscells treated with herbicides DCMU and BNT and exposed to high irradiance,” Photosynthetica, vol. 35, no. 3, pp. 477–480, 1998.
- D. Laval-Martin, G. Dubertret, and R. Calvayrac, “Photosynthetic properties of a DCMU resistant strain of Euglena gracilis Z.,” Plant Science Letters, vol. 10, no. 2, pp. 185–195, 1977.
- Z. Xiankong, K. Haskell, J. B. Tabita, F. R. Tabita, and C. Van Baalen, “Aerobic hydrogen production by the heterocystous cyanobacteria Anabaena spp. strains CA and 1F,” Journal of Bacteriology, vol. 156, no. 3, pp. 1118–1122, 1983.
- M. Chen, Z. Zhang, C. Wang et al., “Improving conversion efficiency of solar energy to electricity in cyanobacterial PEMFC by high levels of photo-H2 production,” International Journal of Hydrogen Energy, vol. 38, no. 31, pp. 13556–13563, 2013.
- M. Chen, J. Li, L. Zhang et al., “Auto-flotation of heterocyst enables the efficient production of renewable energy in cyanobacteria,” Scientific Reports, vol. 4, Article ID 3998, 2014.
- E. Padan, B. Raboy, and M. Shilo, “Endogenous dark respiration of the blue-green alga, Plectonema boryanum,” Journal of Bacteriology, vol. 106, no. 1, pp. 45–50, 1971.
- M. L. Yallop, “Some effects of light on algal respiration and the validity of the light and dark bottle technique for measuring primary productivity,” Freshwater Biology, vol. 12, no. 5, pp. 427–433, 1982.
- R. Rippka, J. Deruelles, J. B. Waterbury, M. Herdman, and R. Y. Stanier, “Generic assignments, strain histories and properties of pure cultures of cyanobacteria,” Journal of General Microbiology, vol. 111, no. 1, pp. 1–61, 1979.
- S. Garlick, A. Oren, and E. Padan, “Occurrence of facultative anoxygenic photosynthesis among filamentous and unicellular cyanobacteria,” Journal of Bacteriology, vol. 129, no. 2, pp. 623–629, 1977.
- G. Mackinney, “Absorption of light by chlorophyll solutions,” The Journal of Biological Chemistry, vol. 140, no. 2, pp. 315–322, 1941.
- S. Joshua, S. Bailey, N. H. Mann, and C. W. Mullineaux, “Involvement of phycobilisome diffusion in energy quenching in cyanobacteria,” Plant Physiology, vol. 138, no. 3, pp. 1577–1585, 2005.
- T. O. I. V. O. Kallas and R. W. Castenholz, “Rapid transient growth at low pH in the cyanobacterium Synechococcus sp,” Journal of Bacteriology, vol. 149, no. 1, pp. 237–246, 1982.
- J. Mottley and D. E. Griffiths, “Minimum inhibitory concentration of a broad range of inhibitors for the unicellular alga Chlamydomonas reinhardi dangeard,” Journal of General Microbiology, vol. 102, no. 2, pp. 431–434, 1977.
- E. H. Burrows, F. W. R. Chaplen, and R. L. Ely, “Effects of selected electron transport chain inhibitors on 24-h hydrogen production by Synechocystis sp. PCC 6803,” Bioresource Technology, vol. 102, no. 3, pp. 3062–3070, 2011.
- A. R. Orr, J. E. Kessler, and E. R. TePaske, “DCMU induced inhibition of growth, photosynthesis and motility in eudorina elegans cultures,” American Journal of Botany, vol. 63, no. 7, pp. 973–978, 1976.
- Y. Gonen-Zurgil, Y. Carmeli-Schwartz, and A. Sukenik, “Selective effect of the herbicide DCMU on unicellular algae – a potential tool to maintain monoalgal mass culture of Nannochloropsis,” Journal of Applied Phycology, vol. 8, no. 4-5, pp. 415–419, 1996.
- D. Gadkari, “Effect of some photosynthesis‐inhibiting herbicides on growth and nitrogenase activity of a new isolate of cyanobacteria, Nostoc G3,” Journal of Basic Microbiology, vol. 28, no. 7, pp. 419–426, 1988.
- S. Singh, P. Datta, and A. Tirkey, “Response of multiple herbicide resistant strain of diazotrophic cyanobacterium, Anabaena variabilis, exposed to atrazine and DCMU,” Indian Journal of Experimental Biology (IJEB), vol. 49, no. 4, pp. 298–303, 2011.
- C.-F. Ji, X.-J. Yu, Z.-A. Chen, S. Xue, J. Legrand, and W. Zhang, “Effects of nutrient deprivation on biochemical compositions and photo-hydrogen production of Tetraselmis subcordiformis,” International Journal of Hydrogen Energy, vol. 36, no. 10, pp. 5817–5821, 2011.
- S. K. Herbert, D. C. Fork, and S. Malkin, “Photoacoustic measurements in Vivo of energy storage by cyclic electron flow in algae and higher plants,” Plant Physiology, vol. 94, no. 3, pp. 926–934, 1990.
- W. Khetkorn, W. Baebprasert, P. Lindblad, and A. Incharoensakdi, “Redirecting the electron flow towards the nitrogenase and bidirectional Hox-hydrogenase by using specific inhibitors results in enhanced H2 production in the cyanobacterium Anabaena siamensis TISTR 8012,” Bioresource Technology, vol. 118, pp. 265–271, 2012.
- W. P. Williams and P. J. Dominy, “Control of excitation energy distribution in cyanobacteria: sensitivity to uncouplers and ATP synthase inhibitors,” BBA - Bioenergetics, vol. 1015, no. 1, pp. 121–130, 1990.
- L. Cournac, F. Mus, L. Bernard, G. Guedeney, P. M. Vignais, and G. Peltier, “Limiting steps of hydrogen production in Chlamydomonas reinhardtii, Synechocystis sp. PCC 6803 as analysed by light-induced gas exchange transients,” International Journal of Hydrogen Energy, vol. 27, no. 11-12, pp. 1229–1237, 2002.
- L. Cournac, G. Guedeney, G. Peltier, and P. M. Vignais, “Sustained photoevolution of molecular hydrogen in a mutant of Synechocystis sp. strain PCC 6803 deficient in the type I NADPH-dehydrogenase complex,” Journal of Bacteriology, vol. 186, no. 13, pp. 1737–1746, 2004.
- F. Koenig, “A role of the QB binding protein in the mechanism of cyanobacterial adaptation to light intensity?” Zeitschrift für Naturforschung C, vol. 42, no. 6, pp. 727–732, 1987.
- D. F. Sargent and C. P. S. Taylor, “Light-induced inhibition of respiration in DCMU-poisoned Chlorella caused by photosystem I activity,” Canadian Journal of Botany, vol. 50, no. 1, pp. 13–21, 1972.
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