RASGRP2 Suppresses Apoptosis via Inhibition of ROS Production in Vascular Endothelial Cells

Figure 3

Effect of ROS inhibitors on TNF-α stimulation. Cells were treated with 20 ng/mL TNF-α for either 24 h or 48 h. Cell viability at 0 h was taken as 100%. (a) and (b) represent the cell viability of the mock cells and TERT HUVEC R cells pretreated with 5 mM NAC, respectively. Blue circle: untreated; red cross: TNF-α; green asterisk: NAC; purple square: TNF-α + NAC. (c) and (d) represent the cell viability of mock cells and TERT HUVEC R cells pretreated with 20 μM DPI, respectively. Blue circle: untreated; red cross: TNF-α; green asterisk: DPI; purple square: TNF-α + DPI. (e) and (f) represent the cell viability of mock cells and TERT HUVEC R cells pretreated with 30 μM apocynin, respectively. Blue circle: untreated; red cross: TNF-α; green asterisk: apocynin; purple square: TNF-α + apocynin. n=3; A = P<0.01, untreated versus TNF-α; B = P<0.01, TNF-α versus TNF-α + NAC; C = P<0.01, TNF-α versus TNF-α + DPI; D = P<0.01, TNF-α versus TNF-α + apocynin.

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