Research Article

Determination of Cassiarin A Level of Cassia siamea Leaf Obtained from Various Regions in Indonesia Using the TLC-Densitometry Method

Table 2

Validation of the TLC-densitometry method of Cassiarin A in C. siamea leaf ethyl acetate fraction.

Parameters

Selectivity (Rs)2.20 (chloroform : ethanol = 85 : 15 v/v)
Linearity (r)0.9928
Accuracy (% recovery)102.81–120.14
Precision (% RSD)1.42–3.95
Detection limit (μg/spot)0.0027
Quantitation limit (μg/spot)0.0080