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Veterinary Medicine International
Volume 2010 (2010), Article ID 149373, 6 pages
Research Article

Immunoprotective Effect of Seabuckthorn (Hippophae rhamnoides) and Glucomannan on T-2 Toxin-Induced Immunodepression in Poultry

1Department of Pharmacology and Toxicology, College of Veterinary and Animal Sciences, CSKHPKV, Himachal Pradesh Palampur 176062, India
2Department of Veterinary Microbiology and Immunology, College of Veterinary and Animal Sciences, CSKHPKV, Himachal Pradesh Palampur 176062, India

Received 21 July 2010; Revised 11 October 2010; Accepted 20 October 2010

Academic Editor: William Ravis

Copyright © 2010 T. Ramasamy et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The present investigation was undertaken to study the immunoprotective effect of seabuckthorn berries and glucomannan against T-2 toxin-induced immunodepression in 15-day-old chicks. T-2 toxin was produced in the laboratory by growing Fusarium sporotrichioides MTCC 2081 on wheat. T-2 toxin was fed to birds at 1 ppm level of the diet. The powdered seabuckthorn berries were added at 400 and 800 ppm levels, and glucomannan added at 1 g/kg of feed. All the treatments were continued up to 28 days. The immunoprotective effects of seabuckthorn and glucomannan were assessed by evaluating humoral immune reaction against NCD vaccine (haemagglutination test and immunoglobulin estimation), serum immunoglobulin levels, phagocytic index, and DTH reaction against DNFB between day 25 and day 28 of experiment. There was significant ( 𝑃 < . 0 5 ) decrease in non-specific immunity in T-2 toxin-treated group as evidenced by a reduction in phagocytic index, DTH reaction, HI titer, and total serum Ig compared to the healthy control group. A significant increase ( 𝑃 < . 0 5 ) in HI titer and total serum Ig was seen in seabuckthorn and glucomannan fed group. A significant ( 𝑃 < . 0 5 ) increase in DTH reaction and non-specific immune response was seen in seabuckthorn and glucomannan fed birds. The present investigation revealed that the seabuckthorn alone protected the immunosuppressant action of T-2 toxin, but seabuckthorn and glucomannan in combination provided an additive protection against T-2 toxicity.