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Veterinary Medicine International
Volume 2010 (2010), Article ID 659862, 7 pages
http://dx.doi.org/10.4061/2010/659862
Research Article

Stallion Sperm Viability, as Measured by the Nucleocounter SP-100, Is Affected by Extender and Enhanced by Single Layer Centrifugation

1Division of Reproduction, Department of Clinical Sciences, Swedish University of Agricultural Sciences (SLU), P.O. Box 7054, 75007 Uppsala, Sweden
2Department of Anatomy, Physiology & Biochemistry, Swedish University of Agricultural Sciences (SLU), P.O. Box 7011, 75007 Uppsala, Sweden

Received 22 June 2009; Accepted 24 August 2009

Academic Editor: Robert J. Kemppainen

Copyright © 2010 J. M. Morrell et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

On-stud assessment of stallion sperm quality can be problematic. A new instrument, the Nucleocounter SP-100, was validated for measuring stallion sperm concentration and viability. It was subsequently used to evaluate sperm viability in Kenney's extender and INRA96. There was a strong correlation between sperm concentrations measured by the Nucleocounter SP-100 and by the Bürker counting chamber ( 𝑟 = 0 . 8 4 ; 𝑃 < . 0 0 1 ). Similarly, there was a good correlation between sperm viability results from the Nucleocounter SP-100 and flow cytometric results ( 𝑟 = 0 . 7 3 ; 𝑃 < . 0 0 1 ). Sperm viability at 24 hours was significantly better for samples extended in INRA96 than in Kenney's extender ( 𝑃 < . 0 0 1 ). Furthermore, sperm kinematics were better for stored samples in INRA96 than in Kenney's extender. Single Layer Centrifugation selected spermatozoa that maintained their viability better during storage for 24 hours than the uncentrifuged samples. In conclusion, the type of semen extender used and Single Layer Centrifugation were found to influence both the kinematics and viability of stallion spermatozoa. The Nucelocounter-SP100 was considered to be a useful instrument for rapidly measuring stallion sperm concentration and viability.