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Veterinary Medicine International
Volume 2010, Article ID 659862, 7 pages
http://dx.doi.org/10.4061/2010/659862
Research Article

Stallion Sperm Viability, as Measured by the Nucleocounter SP-100, Is Affected by Extender and Enhanced by Single Layer Centrifugation

1Division of Reproduction, Department of Clinical Sciences, Swedish University of Agricultural Sciences (SLU), P.O. Box 7054, 75007 Uppsala, Sweden
2Department of Anatomy, Physiology & Biochemistry, Swedish University of Agricultural Sciences (SLU), P.O. Box 7011, 75007 Uppsala, Sweden

Received 22 June 2009; Accepted 24 August 2009

Academic Editor: Robert J. Kemppainen

Copyright © 2010 J. M. Morrell et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Citations to this Article [23 citations]

The following is the list of published articles that have cited the current article.

  • Morrell, Rodriguez-Martinez, and Johannisson, “Single layer centrifugation of stallion spermatozoa improves sperm quality compared with sperm washing,” Reproductive BioMedicine Online, vol. 21, no. 3, pp. 429–436, 2010. View at PublisherView at Google Scholar
  • Morrell, Rodriguez-Martinez, and Johannisson, “Single layer centrifugation of stallion spermatozoa consistently selects the most robust spermatozoa from the rest of the ejaculate in a large sample size,” Equine Veterinary Journal, vol. 42, no. 7, pp. 579–585, 2010. View at PublisherView at Google Scholar
  • J. M. Morrell, and M. Wallgren, “Colloid Centrifugation of Boar Semen,” Reproduction In Domestic Animals, vol. 46, pp. 18–22, 2011. View at PublisherView at Google Scholar
  • J. M. Morrell, G. Mari, G. Kutvolgyi, S. Meurling, B. Mislei, E. Iacono, and H. Rodriguez-Martinez, “Pregnancies Following Artificial Insemination with Spermatozoa from Problem Stallion Ejaculates Processed by Single Layer Centrifugation with Androcoll-E,” Reproduction in Domestic Animals, vol. 46, no. 4, pp. 642–645, 2011. View at PublisherView at Google Scholar
  • Cristina Ortega-Ferrusola, Anders Johannisson, Fernando J. Pena Vega, Jose A. Tapia, Heriberto Rodriguez-Martinez, Ann M. Dalin, and Jane M. Morrell, “Effect of Different Extenders and Seminal Plasma on the Susceptibility of Equine Spermatozoa to Lipid Peroxidation After Single-Layer Centrifugation, Through Androcoll-E,” Journal Of Equine Veterinary Science, vol. 31, no. 7, pp. 411–416, 2011. View at PublisherView at Google Scholar
  • M. L. Foster, C. C. Love, D. D. Varner, S. P. Brinsko, K. Hinrichs, S. Teague, K. LaCaze, and T. L. Blanchard, “Comparison of methods for assessing integrity of equine sperm membranes,” Theriogenology, vol. 76, no. 2, pp. 334–341, 2011. View at PublisherView at Google Scholar
  • Morrell, B. Macias Garcia, Pena, and Johannisson, “Processing stored stallion semen doses by Single Layer Centrifugation,” Theriogenology, vol. 76, no. 8, pp. 1424–1432, 2011. View at PublisherView at Google Scholar
  • Hoogewijs, Morrell, Van Soom, Govaere, Johannisson, Piepers, De Schauwer, De Kruif, and De Vliegher, “Sperm selection using single layer centrifugation prior to cryopreservation can increase thawed sperm quality in stallions,” Equine Veterinary Journal, vol. 43, no. 40, pp. 35–41, 2011. View at PublisherView at Google Scholar
  • M. K. Hoogewijs, S. P. De Vliegher, J. L. Govaere, C. De Schauwer, A. De Kruif, and A. Van Soom, “Influence of counting chamber type on CASA outcomes of equine semen analysis,” Equine Veterinary Journal, vol. 44, no. 5, pp. 542–549, 2012. View at PublisherView at Google Scholar
  • Johanna Lindahl, Anne-Marie Dalin, Gesa Stuhtmann, and Jane M. Morrell, “Stallion spermatozoa selected by single layer centrifugation are capable of fertilization after storage for up to 96 h at 6 degrees C prior to artificial insemination,” Acta Veterinaria Scandinavica, vol. 54, 2012. View at PublisherView at Google Scholar
  • J. M. Morrell, J. Pihl, A-M Dalin, and A. Johannisson, “Restoration of seminal plasma to stallion spermatozoa selected by colloid centrifugation increases sperm progressive motility but is detrimental to chromatin integrity,” Theriogenology, vol. 78, no. 2, pp. 345–352, 2012. View at PublisherView at Google Scholar
  • H. Rodriguez-Martinez, “Assisted Reproductive Techniques for Cattle Breeding in Developing Countries: A Critical Appraisal of Their Value and Limitations,” Reproduction In Domestic Animals, vol. 47, pp. 21–26, 2012. View at PublisherView at Google Scholar
  • C. Winblad, A. Johannisson, G. Stuhtmann, J. M. Morrell, A. Georgakas, and P. Humblot, “Reactive oxygen species in stallion semen can be affected by season and colloid centrifugation,” Animal Reproduction Science, vol. 140, no. 1-2, pp. 62–69, 2013. View at PublisherView at Google Scholar
  • J.M. Morrell, A. Georgakis, N. Lundeheim, D. Nash, Davies Morel, and A. Johannisson, “Effect of heterologous and homologous seminal plasma on stallion sperm quality,” Theriogenology, 2014. View at PublisherView at Google Scholar
  • Korochkina, Johannisson, Lavanya Goodla, Morrell, and Axner, “Effect of prostatic fluid on the quality of fresh and frozen-thawed canine epididymal spermatozoa,” Theriogenology, vol. 82, no. 9, pp. 1206–1211, 2014. View at PublisherView at Google Scholar
  • J.M. Morrell, G. Stuhtmann, S. Meurling, A. Lundgren, C. Winblad, B. Macias Garcia, and A. Johannisson, “Sperm yield after Single Layer Centrifugation with Androcoll-E is related to the potential fertility of the original ejaculate,” Theriogenology, 2014. View at PublisherView at Google Scholar
  • J. M. Morrell, and A. Johannisson, “Comparison of the Effect of Heterologous and Homologous Seminal Plasma on Motility and Chromatin Integrity of Stallion Spermatozoa Selected by Single Layer Centrifugation,” Journal of Veterinary Medicine, vol. 2014, pp. 1–6, 2014. View at PublisherView at Google Scholar
  • I. Ortiz, J. Dorado, J.M. Morrell, F. Crespo, J. Gosálvez, M.J. Gálvez, D. Acha, and M. Hidalgo, “Effect of single-layer centrifugation or washing on frozen–thawed donkey semen quality: Do they have the same effect regardless of the quality of the sample?,” Theriogenology, 2015. View at PublisherView at Google Scholar
  • A. Vicente-Carrillo, I. Edebert, H. Garside, I. Cotgreave, R. Rigler, V. Loitto, K.E. Magnusson, and H. Rodríguez-Martínez, “Boar spermatozoa successfully predict mitochondrial modes of toxicity: Implications for drug toxicity testing and the 3R principles,” Toxicology in Vitro, 2015. View at PublisherView at Google Scholar
  • L. Daub, A. Geyer, S. Reese, J. Braun, and C. Otzdorff, “Sperm membrane integrity in fresh and frozen-thawed canine semen samples: A comparison of vital stains with the NucleoCounter SP-100®,” Theriogenology, 2016. View at PublisherView at Google Scholar
  • J. M. Morrell, A. Lagerqvist, P. Humblot, and A. Johannisson, “Effect of Single Layer Centrifugation on reactive oxygen species and sperm mitochondrial membrane potential in cooled stallion semen,” Reproduction, Fertility and Development, vol. 29, no. 5, pp. 1039, 2017. View at PublisherView at Google Scholar
  • E. Varela, E. Plaza, P. Muñoz de propios, J.M. Ortiz-Rodríguez, M. Álvarez, L. Anel-López, L. Anel, P. De Paz, M.C. Gil, J. Rey, J.M. Morrell, and C. Ortega-Ferrusola, “How does the microbial load affect the quality of equine cool-stored semen?,” Theriogenology, 2018. View at PublisherView at Google Scholar
  • William V. Holt, “Is the Classic Spermiogram Still Informative? How Did It Develop and Where Is It Going?,” Reproductomics, pp. 1–19, 2018. View at PublisherView at Google Scholar