Stallion Sperm Viability, as Measured by the Nucleocounter SP-100, Is Affected by Extender and Enhanced by Single Layer Centrifugation

Figure 3

Mean values for total motility (%) and progressive motility (%) for one stallion (Stallion I) $(n=4)$. Note: SLC: Single Layer Centrifugation; I: INRA96; K: Kenney’s extender. Significant differences and trends towards significance between 0 hour and 24 hours within each treatment group are shown on the figure. Other significant differences were found as follows: for progressive motility between $\text{K}$ uncentrifuged and $\text{K}$ SLC at 24 hours $(P<.05)$, between $\text{I}$ uncentrifuged and $\text{I}$ SLC at 24 hours $(P<.001)$, and between $\text{K}$ SLC and $\text{I}$ SLC at 0 hour $(P<.05)$; for total motility, between $\text{K}$ uncentrifuged and K SLC at 24 hours $(P<.05)$, and between $\text{I}$ uncentrifuged and $\text{I}$ SLC at 24 hours $(P<.01)$. There were no differences between ejaculates except for total motility, uncentrifuged, $\text{I}$ versus $\text{K}$ at 0 hour.