Stallion Sperm Viability, as Measured by the Nucleocounter SP-100, Is Affected by Extender and Enhanced by Single Layer Centrifugation

Table 1

Mean ($\pm$SD) values for sperm kinematics of cool-stored stallion spermatozoa in different extenders after Single Layer Centrifugation $(n=45)$.

Parameter	Time	Uncentrifuged $\text{I}$	Centrifuged $\text{I}$	Uncentrifuged $\text{K}$	Centrifuged $\text{K}$
Total motility (%)	0 hour	$76.4\pm {12.4}^{\text{b}}$	$83.8\pm {8.9}^{\text{b}}$	$74.8\pm 12.4$	$77.1\pm 14.3$
	24 hours	$63.0\pm {15.5}^{\text{ab}}$	$78.9\pm {13.9}^{\text{b}}$	$56.4\pm {17.2}^{\text{ac}}$	$65.1\pm {19.7}^{\text{c}}$
Progressive motility (%)	0 hour	$54.7\pm {17}^{\text{b}}$	$67.3\pm {16.5}^{\text{b}}$	$52.6\pm 15.7$	$56.8\pm 18.2$
	24 hours	$40.2\pm {16}^{\text{ab}}$	$64.5\pm {16.7}^{\text{b}}$	$30.8\pm {12.5}^{\text{ac}}$	$42.7\pm {21}^{\text{c}}$
Velocity ($\mu \text{m}$/s)	0 hours	$48.3\pm {14.8}^{\text{b}}$	$43.7\pm {14.2}^{\text{b}}$	$46.3\pm 14$	$40.6\pm 13$
	24 hours	$38.1\pm {15.6}^{\text{ab}}\mathrm{\hspace{0.17em}\hspace{0.17em}}$	$49.6\pm {14.5}^{\text{b}}$	$28\pm {11.5}^{\text{ac}}$	$36.9\pm {16.5}^{\text{c}}$

Note: I:INRA96, K: Kenney extender. a: significant difference between INRA96 and Kenney’s extender for uncentrifuged samples; b: significant difference between uncentrifuged and centrifuged samples in INRA96; c: significant difference between uncentrifuged and centrifuged samples in Kenney’s extender.