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Veterinary Medicine International
Volume 2010, Article ID 748621, 8 pages
http://dx.doi.org/10.4061/2010/748621
Research Article

Diagnostic Application of IS900 PCR Using Blood as a Source Sample for the Detection of Mycobacterium avium Subspecies Paratuberculosis in Early and Subclinical Cases of Caprine Paratuberculosis

Microbiology Laboratory, Animal Health Division, Central Institute for Research on Goats, Makhdoom, PO - Farah, Mathura (UP), Uttar Pradesh 281 122, India

Received 27 May 2009; Revised 6 July 2009; Accepted 26 August 2009

Academic Editor: John Hopkins

Copyright © 2010 P. K. Singh et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Citations to this Article [19 citations]

The following is the list of published articles that have cited the current article.

  • Katrina L. Bower, Douglas J. Begg, and Richard J. Whittington, “Culture of Mycobacterium avium subspecies paratuberculosis (MAP) from blood and extra-intestinal tissues in experimentally infected sheep,” Veterinary Microbiology, vol. 147, no. 1-2, pp. 127–132, 2011. View at Publisher · View at Google Scholar
  • P. K. Singh, S. V. Singh, M. K. Singh, V. K. Saxena, P. Horin, A. V. Singh, and J. S. Sohal, “Effect of genetic variation in the MHC Class II DRB region on resistance and susceptibility to Johne's disease in endangered Indian Jamunapari goats,” International Journal Of Immunogenetics, vol. 39, no. 4, pp. 314–320, 2012. View at Publisher · View at Google Scholar
  • Sulficar Shamsudeen Rawther, Mapranath Raghavan Saseendranath, Govinda Pilla Krishnan Nair, Palathingal Vareid Tresamol, Usha Narayana Pillai, John Abraham, Tuticorin Maragatham Alagesan Senthilkumar, Shrine Nagalakshmy, and Krishna Kripa Narayanan Nimisha, “Diagnosis of paratuberculosis in goats by cell mediated immune response, co nventional and molecular diagnostic techniques,” Tropical Animal Health and Production, vol. 44, no. 4, pp. 911–914, 2012. View at Publisher · View at Google Scholar
  • K. Singh, B. S. Chandel, H. C. Chauhan, A. Dadawala, S. V. Singh, and P. K. Singh, “Efficacy of 'indigenous vaccine' using native 'Indian bison type' genotype of Mycobacterium avium subspecies paratuberculosis for the control of clinical Johne's disease in an organized goat herd,” Veterinary Research Communications, vol. 37, no. 2, pp. 109–114, 2013. View at Publisher · View at Google Scholar
  • Katrina L. Bower, Douglas J. Begg, and Richard J. Whittington, “Tissue localisation of Mycobacterium avium subspecies paratuberculosis foll owing artificially induced intracellular and naked bacteraemia,” Veterinary Microbiology, vol. 162, no. 1, pp. 112–118, 2013. View at Publisher · View at Google Scholar
  • Singh, Singh, Saxena, Singh, Singh, and Sohal, “Expression Profiles of Different Cytokine Genes in Peripheral Blood Mononuclear Cells of Goats Infected Experimentally with Native Strain of Mycobacterium Avium Subsp. Paratuberculosis,” Animal Biotechnology, vol. 24, no. 3, pp. 187–197, 2013. View at Publisher · View at Google Scholar
  • Shoor Vir Singh, Ajay Vir Singh, Pravin Kumar Singh, Saurabh Gupta, Harendra Singh, Brajesh Singh, Omalur Rajendran Vinodh Kumar, Aripudur Sellappan Rajendiran, Narendra Nath Swain, and Jagdip Singh Sohal, “Evaluation of “Indigenous Vaccine” Developed Using “Indian Bison Type” Genotype of <i>Mycobacterium avium subspecies paratuberculosis</i> Strain “S5” of Goat Origin in a Sheep Flock Endemic for Johne’s Disease: A Three Years Trial in India,” World Journal of Vaccines, vol. 03, no. 02, pp. 52–59, 2013. View at Publisher · View at Google Scholar
  • Bharathy Sukumar, Lakshmanasami Gunaseelan, Kannan Porteen, and Karuppanasamy Prabu, “Goat milk as a non-invasive sample for confirmation of Mycobacterium avium subspecies paratuberculosis by IS900 PCR,” Journal of Advanced Veterinary and Animal Research, vol. 1, no. 3, pp. 136–139, 2014. View at Publisher · View at Google Scholar
  • G. S. Abdellrazeq, M. M. El-Naggar, S. A. Khaliel, and A. E. Gamal-Eldin, “Detection of Mycobacterium avium subsp. paratuberculosis from cattle and buffaloes in Egypt using traditional culture, serological and molecular based methods,” Veterinary World, vol. 7, no. 8, pp. 586–593, 2014. View at Publisher · View at Google Scholar
  • Karren M. Plain, Auriol C. Purdie, Kumudika de Silva, Douglas Begg, Richard J. Whittington, and Shyamala Thirunavukkarasu, “Expression of genes associated with cholesterol and lipid metabolism identified as a novel pathway in the early pathogenesis of Mycobacterium avium subspecies paratuberculosis-infection in cattle,” Veterinary Immunology and Immunopathology, vol. 160, no. 3-4, pp. 147–157, 2014. View at Publisher · View at Google Scholar
  • Barad, Singh, Sohal, Gupta, Chaubey, Chakraborty, Tiwari, Deb, Dhama, Chandel, Dadawala, Chauhan, Kher, Shroff, Bhagat, Singh, and Singh, “Incidence of Mycobacterium Avium subspecies paratuberculosis in mehsani and surti goats of Indian origin using multiple diagnostic tests,” Journal of Biological Sciences, vol. 14, no. 2, pp. 124–133, 2014. View at Publisher · View at Google Scholar
  • Kumar, Dhama, Singh, Srivastava, Gangwar, Singh, Gupta, Chaubey, Tiwari, and Chakraborty, “Comparative evaluation of 'indigenous' and commercial vaccines in double challenge model for the control of caprine paratuberculosis in India,” Journal of Biological Sciences, vol. 14, no. 3, pp. 169–182, 2014. View at Publisher · View at Google Scholar
  • Shoor Vir Singh, Kuldeep Dhama, Saurabh Gupta, Kundan Kumar Chaubey, Krishna Dutta Rawat, Naveen Kumar, Jagdip Singh Sohal, Sarjeet Singh, Ruchi Tiwari, and Sandip Chakraborty, “Johne's disease (JD) in a high yielding holstein friesian cattle dairy farm in India,” Journal of Biological Sciences, vol. 14, no. 3, pp. 195–203, 2014. View at Publisher · View at Google Scholar
  • Shoor Vir Singh, Krishna Dutta Rawat, Rajib Deb, Kuldeep Dhama, Naveen Kumar, Jagdip Singh Sohal, Ajay Vir Singh, Pravin Kumar Singh, Narottam Das Agrawal, Saurabh Gupta, Kundan Kumar Chaubey, and Anvish Kumar, “First mass screening of the human population to estimate the bio-load of mycobacterium avium subspecies paratuberculosis in north India,” Journal of Biological Sciences, vol. 14, no. 4, pp. 237–247, 2014. View at Publisher · View at Google Scholar
  • Mohamed Sabry, and Ahmed Elkerdasy, “A polymerase chain reaction and enzyme linked immunosorbent assay based approach for diagnosis and differentiation between vaccinated and infected cattle with Mycobacterium bovis,” Journal of Pharmacy and Bioallied Sciences, vol. 6, no. 2, pp. 115–121, 2014. View at Publisher · View at Google Scholar
  • Theonys Diógenes Freitas, Sérgio Santos De Azevedo, Maria Luana Cristiny Rodrigues Silva, Felício Garino, Carolina De Sousa Américo Batista Santos, Inácio José Clementino, Franklin Riet-Correa Amaral, and Clebert José Alves, “Epidemiological characterization and risk factors associated with Mycobacterium avium subsp. Paratuberculosis infection in dairy goats in the Brazilian semiarid region,” Semina:Ciencias Agrarias, vol. 36, no. 1, pp. 267–276, 2015. View at Publisher · View at Google Scholar
  • Gurusimiran Filia, Pravin Kumar Singh, Sujata Jayaraman, Saurabh Gupta, Kundan Kumar Chaubey, Ruchi Tiwari, Mani Saminathan, Kuldeep Dhama, Jagdip Singh Sohal, Rajni Garg, Prasanna Kumar Patil, Shoor Vir Singh, Shukriti Sharma, Ravi Kumar Gandham, and Ajay Vir Singh, “ Comparative Evaluation of Different Test Combinations for Diagnosis of Mycobacterium avium Subspecies paratuberculosis Infecting Dairy Herds in India ,” BioMed Research International, vol. 2015, pp. 1–6, 2015. View at Publisher · View at Google Scholar
  • Shobha Rani Methuku, Anuradha Menon Elattuvalappil, Srikanth Mallaiah, Doddamane Rathnamma, Shoorvir Singh, Manjunath Suranagi Shambanna, Shivalingappa Yamanappa Mukartal, Hogalagere Doddappaiah Narayanaswamy, Shrikrishna Isloor, and Basavegowdanadoddi Marinaik Chandranaik, “Prevalence of ovine Johne's disease in bannur breed of sheep in organized farm using multiple diagnostic tests,” Advances in Animal and Veterinary Sciences, vol. 4, no. 10, pp. 506–512, 2016. View at Publisher · View at Google Scholar
  • Shoor Vir Singh, Saurabh Gupta, Kundan Kumar Chau, Saket Bhushan, Krishna Dutta Rawa, Naveen Kumar, Hari Audh Tiwar, Vinay Chaturvedi, Jagdip Singh Soha, Kuldeep Dhama, and Zahra Hemati, “'Therapeutic Management' of Incurable Paratuberculosis Using 'Indigenous Vaccine' in Goatherds, Endemically Infected with Johne's Disease,” International Journal of Pharmacology, vol. 13, no. 2, pp. 145–155, 2017. View at Publisher · View at Google Scholar