Table of Contents Author Guidelines Submit a Manuscript
Veterinary Medicine International
Volume 2010, Article ID 748621, 8 pages
http://dx.doi.org/10.4061/2010/748621
Research Article

Diagnostic Application of IS900 PCR Using Blood as a Source Sample for the Detection of Mycobacterium avium Subspecies Paratuberculosis in Early and Subclinical Cases of Caprine Paratuberculosis

Microbiology Laboratory, Animal Health Division, Central Institute for Research on Goats, Makhdoom, PO - Farah, Mathura (UP), Uttar Pradesh 281 122, India

Received 27 May 2009; Revised 6 July 2009; Accepted 26 August 2009

Academic Editor: John Hopkins

Copyright © 2010 P. K. Singh et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. S. L. Ott, S. J. Wells, and B. A. Wagner, “Herd-level economic losses associated with Johne's disease on US dairy operations,” Preventive Veterinary Medicine, vol. 40, no. 3-4, pp. 179–192, 1999. View at Publisher · View at Google Scholar
  2. S. V. Singh, A. V. Singh, R. Singh et al., “Sero-prevalence of Bovine Johne's disease in buffaloes and cattle population of North India using indigenous ELISA kit based on native Mycobacterium avium subspecies paratuberculosis ‘Bison type’ genotype of goat origin,” Comparative Immunology, Microbiology and Infectious Diseases, vol. 31, no. 5, pp. 419–433, 2008. View at Publisher · View at Google Scholar · View at PubMed
  3. M. Salgado, E. J. B. Manning, and M. T. Collins, “Performance of a Johne's disease enzyme-linked immunosorbent assay adapted for milk samples from goats,” Journal of Veterinary Diagnostic Investigation, vol. 17, no. 4, pp. 350–354, 2005. View at Google Scholar
  4. N. Singh, S. V. Singh, V. K. Gupta, V. D. Sharma, R. K. Sharma, and V. M. Katoch, “Isolation and identification of Mycobacterium paratuberculosis from naturally infected goatherds in India,” Indian Journal of Veterinary Pathology, vol. 20, pp. 104–108, 1996. View at Google Scholar
  5. P. Kumar, S. V. Singh, A. K. Bhatiya et al., “Juvenile Capri-Paratuberculosis (JCP) in India: incidence and characterization by six diagnostic tests,” Small Ruminant Research, vol. 73, no. 1–3, pp. 45–53, 2007. View at Publisher · View at Google Scholar
  6. C. J. Clarke, “The pathology and pathogenesis of paratuberculosis in ruminants and other species,” Journal of Comparative Pathology, vol. 116, no. 3, pp. 217–261, 1997. View at Google Scholar
  7. C. D. Buergelt and J. E. Williams, “Nested PCR on blood and milk for the detection of Mycobacterium avium subsp paratuberculosis DNA in clinical and subclinical bovine paratuberculosis,” Australian Veterinary Journal, vol. 82, no. 8, pp. 497–503, 2004. View at Publisher · View at Google Scholar
  8. S. Kumar, S. V. Singh, I. Sevilla et al., “Lacto-prevalence, genotyping of Mycobacterium avium subspecies paratuberculosis and evaluation of three diagnostic tests in milk of naturally infected goatherds,” Small Ruminant Research, vol. 74, no. 1–3, pp. 37–44, 2008. View at Publisher · View at Google Scholar
  9. I. W. Lugton, “Mucosa-associated lymphoid tissues as sites for uptake, carriage and excretion of tubercle bacilli and other pathogenic mycobacteria,” Immunology and Cell Biology, vol. 77, no. 4, pp. 364–372, 1999. View at Publisher · View at Google Scholar · View at PubMed
  10. S. Hajra, S. V. Singh, and A. K. Srivastava, “Pathobiology of spontaneous and experimental paratuberculosis (S-5 strain) in goats with special reference to early lesion,” in Proceedings of the 8th International Colloquium on Paratuberculosis, E. J. B. Manning and S. S. Nielsen, Eds., p. 31, Copenhagen, Denmark, August 2005.
  11. D. M. Collins, F. Hilbink, D. M. West, B. D. Hosie, M. M. Cooke, and G. W. de Lisle, “Investigation of Mycobacterium paratuberculosis in sheep by faecal culture, DNA characterisation and the polymerase chain reaction,” Veterinary Record, vol. 133, no. 24, pp. 599–600, 1993. View at Google Scholar
  12. R. A. Juste, J. M. Garrido, M. Geijo et al., “Comparison of blood polymerase chain reaction and enzyme-linked immunosorbent assay for detection of Mycobacterium avium subsp. paratuberculosis infection in cattle and sheep,” Journal of Veterinary Diagnostic Investigation, vol. 17, no. 4, pp. 354–359, 2005. View at Google Scholar
  13. J. Vohra, S. V. Singh, A. V. Singh, P. K. Singh, and J. S. Sohal, “Comparative distribution of Mycobacterium avium subsp paratuberculosis in target and non-target tissues of goats and sheep population in India,” Indian Journal of Animal Sciences, vol. 78, pp. 4–10, 2008. View at Google Scholar
  14. N. B. Harris and R. G. Barletta, “Mycobacterium avium subsp. paratuberculosis in Veterinary medicine,” Clinical Microbiology Reviews, vol. 14, no. 3, pp. 489–512, 2001. View at Publisher · View at Google Scholar · View at PubMed
  15. S. A. Naser, G. Ghobrial, C. Romero, and J. F. Valentine, “Culture of Mycobacterium avium subspecies paratuberculosis from the blood of patients with Crohn's disease,” The Lancet, vol. 364, no. 9439, pp. 1039–1044, 2004. View at Publisher · View at Google Scholar · View at PubMed
  16. A. W. Stott, G. M. Jones, R. W. Humphry, and G. J. Gunn, “Financial incentive to control paratuberculosis (Johne's disease) on dairy farms in the United Kingdom,” Veterinary Record, vol. 156, no. 26, pp. 825–831, 2005. View at Google Scholar
  17. M. Bhide, E. Chakurkar, L. Tkacikova, S. Barbuddhe, M. Novak, and I. Mikula, “IS900-PCR-based detection and characterization of Mycobacterium avium subsp. paratuberculosis from buffy coat of cattle and sheep,” Veterinary Microbiology, vol. 112, no. 1, pp. 33–41, 2006. View at Publisher · View at Google Scholar · View at PubMed
  18. S. Englund, G. Bölske, and K.-E. Johansson, “An IS900-like sequence found in a Mycobacterium sp. other than Mycobacterium avium subsp. paratuberculosis,” FEMS Microbiology Letters, vol. 209, no. 2, pp. 267–271, 2002. View at Publisher · View at Google Scholar
  19. C. Coetsier, P. Vannuffel, N. Blondeel, J.-F. Denef, C. Cocito, and J.-L. Gala, “Duplex PCR for differential identification of Mycobactelium bovis, M. avium, and M. avium subsp. paratuberculosis in formalin-fixed paraffin-embedded tissues from cattle,” Journal of Clinical Microbiology, vol. 38, no. 8, pp. 3048–3054, 2000. View at Google Scholar
  20. B. Strommenger, K. Stevenson, and G.-F. Gerlach, “Isolation and diagnostic potential of ISMav2, a novel insertion sequence-like element from Mycobacterium avium subspecies paratuberculosis,” FEMS Microbiology Letters, vol. 196, no. 1, pp. 31–37, 2001. View at Publisher · View at Google Scholar
  21. D. Rodríguez-Lázaro, J. Lloyd, A. Herrewegh et al., “A molecular beacon-based real-time NASBA assay for detection of Mycobacterium avium subsp. paratuberculosis in water and milk,” FEMS Microbiology Letters, vol. 237, no. 1, pp. 119–126, 2004. View at Publisher · View at Google Scholar · View at PubMed
  22. L. Li, J. P. Bannantine, Q. Zhang et al., “The complete genome sequence of Mycobacterium avium subspecies paratuberculosis,” Proceedings of the National Academy of Sciences of the United States of America, vol. 102, no. 35, pp. 12344–12349, 2005. View at Publisher · View at Google Scholar · View at PubMed
  23. N. Singh, S. N. Kala, V. S. Vihan, and S. V. Singh, “Genetic study on the susceptibility to Johne's disease in goats,” Indian Journal of Animal Sciences, vol. 60, pp. 1163–1165, 1990. View at Google Scholar
  24. P. K. Singh, S. V. Singh, A. V. Singh, and J. S. Singh, “Variability in susceptibility of different Indian goat breeds with respect to natural and experimental infection of Mycobacterium avium subsp. paratuberculosis,” Indian Journal of Small Ruminants, vol. 15, pp. 35–43, 2009. View at Google Scholar
  25. D. S. Millar, S. J. Withey, M. L. V. Tizard, J. G. Ford, and J. Hermon-Taylor, “Solid-phase hybridization capture of low-abundance target DNA sequences: application to the polymerase chain reaction detection of Mycobacterium paratuberculosis and Mycobacterium avium subsp. silvaticum,” Analytical Biochemistry, vol. 226, no. 2, pp. 325–330, 1995. View at Publisher · View at Google Scholar
  26. I. Sevilla, S. V. Singh, J. M. Garrido et al., “Molecular typing of Mycobacterium avium subspecies paratuberculosis strains from different hosts and regions,” Revue Scientifique et Technique, vol. 24, no. 3, pp. 1061–1066, 2005. View at Google Scholar
  27. R. J. Whittington, I. B. Marsh, and R. H. Whitlock, “Typing of IS1311 polymorphisms confirms that bison (Bison bison) with paratuberculosis in Montana are infected with a strain of Mycobacterium avium subsp. paratuberculosis distinct from that occurring in cattle and other domesticated livestock,” Molecular and Cellular Probes, vol. 15, no. 3, pp. 139–145, 2001. View at Publisher · View at Google Scholar · View at PubMed
  28. O. H. Lowry, N. J. Rosebrough, A. L. Farr, and R. J. Randall, “Protein measurement with Folin-phenol reagent,” The Journal of Biological Chemistry, vol. 193, pp. 265–276, 1951. View at Google Scholar
  29. M. T. Collins, “Interpretation of a commercial bovine paratuberculosis enzyme-linked immunosorbent assay by using likelihood ratios,” Clinical and Diagnostic Laboratory Immunology, vol. 9, no. 6, pp. 1367–1371, 2002. View at Publisher · View at Google Scholar
  30. F. Arizmendi and J. E. Grimes, “Comparison of the Gimenez staining method and antigen detection ELISA with culture for detecting Chlamydiae in birds,” Journal of Veterinary Diagnostic Investigation, vol. 7, no. 3, pp. 400–401, 1995. View at Google Scholar
  31. J. R. Landis and G. G. Koch, “The measurement of observer agreement for categorical data,” Biometrics, vol. 33, no. 1, pp. 159–174, 1977. View at Google Scholar
  32. S. V. Singh, A. V. Singh, P. K. Singh, J. S. Sohal, and N. P. Singh, “Evaluation of an indigenous ELISA for diagnosis of Johne's disease and its comparison with commercial kits,” Indian Journal of Microbiology, vol. 47, no. 3, pp. 251–258, 2007. View at Publisher · View at Google Scholar
  33. C. Burrells, C. J. Clarke, A. Colston et al., “A study of immunological responses of sheep clinically-affected with paratuberculosis (Johne's disease). The relationship of blood, mesenteric lymph node and intestinal lymphocyte responses to gross and microscopic pathology,” Veterinary Immunology and Immunopathology, vol. 66, no. 3-4, pp. 343–358, 1998. View at Publisher · View at Google Scholar
  34. C. J. Clarke and D. Little, “The pathology of ovine paratuberculosis: gross and histopathological changes in the intestine and other tissues,” The Journal of Comparative Pathology, vol. 114, pp. 410–437, 1996. View at Google Scholar
  35. H. J. Van Kruiningen, R. J. Chiodini, W. R. Thayer, J. A. Coutu, R. S. Merkal, and P. L. Runnels, “Experimental disease in infant goats induced by a Mycobacterium isolated from a patient with Crohn's disease. A preliminary report,” Digestive Diseases and Sciences, vol. 31, no. 12, pp. 1351–1360, 1986. View at Google Scholar
  36. B. G. Zurbrick and C. J. Czuprynski, “Ingestion and intracellular growth of Mycobacterium paratuberculosis within bovine blood monocytes and monocyte-derived macrophages,” Infection and Immunity, vol. 55, no. 7, pp. 1588–1593, 1987. View at Google Scholar
  37. J. M. Gwozdz, K. G. Thompson, B. W. Manktelow, A. Murray, and D. M. West, “Vaccination against paratuberculosis of lambs already infected experimentally with Mycobacterium avium subspecies paratuberculosis,” Australian Veterinary Journal, vol. 78, no. 8, pp. 560–566, 2000. View at Google Scholar
  38. G. M. Barrington, J. M. Gay, I. S. Eriks et al., “Temporal patterns of diagnostic results in serial samples from cattle with advanced paratuberculosis infections,” Journal of Veterinary Diagnostic Investigation, vol. 15, no. 2, pp. 195–200, 2003. View at Google Scholar
  39. W. L. McDonald, S. E. Ridge, A. F. Hope, and R. J. Condron, “Evaluation of diagnostic tests for Johne's disease in young cattle,” Australian Veterinary Journal, vol. 77, no. 2, pp. 113–119, 1999. View at Google Scholar
  40. J. M. Gwozdz, M. P. Reichel, A. Urray et al., “Detection of Mycobacterium avium subspecies paratuberculosis in ovine tissues and blood by the polymerase chain reaction,” Veterinary Microbiology, vol. 51, pp. 233–244, 1997. View at Google Scholar
  41. D. L. Whipple, P. A. Kapke, and P. R. Andersen, “Comparison of a commercial DNA probe test and three cultivation procedures for detection of Mycobacterium paratuberculosis in bovine feces,” Journal of Veterinary Diagnostic Investigation, vol. 4, no. 1, pp. 23–27, 1992. View at Google Scholar
  42. S. E. Seitz, L. E. Heider, W. D. Heuston, S. Bech-Nielsen, D. M. Rings, and L. Spangler, “Bovine fetal infection with Mycobacterium paratuberculosis,” Journal of the American Veterinary Medical Association, vol. 194, no. 10, pp. 1423–1426, 1989. View at Google Scholar
  43. R. W. Sweeney, R. H. Whitlock, and A. E. Rosenberger, “Mycobacterium paratuberculosis cultured from milk and supramammary lymph nodes of infected asymptomatic cows,” Journal of Clinical Microbiology, vol. 30, no. 1, pp. 166–171, 1992. View at Google Scholar
  44. S. V. Singh, Diagnosis of paratuberculosis in goats, Ph.D. thesis, CSA University of Agriculture and Technology, Kanpur, India, 1998.
  45. S. A. Hines, C. D. Buergelt, J. H. Wilson, and E. L. Bliss, “Disseminated Mycobacterium paratuberculosis infection in a cow,” Journal of the American Veterinary Medical Association, vol. 190, no. 6, pp. 681–683, 1987. View at Google Scholar
  46. E. J. B. Manning and M. T. Collins, “Mycobacterium avium subsp. paratuberculosis: pathogen, pathogenesis and diagnosis,” Revue Scientifique et Technique, vol. 20, no. 1, pp. 133–150, 2001. View at Google Scholar
  47. M. Maclure and W. C. Willett, “Misinterpretation and misuse of the kappa statistic,” American Journal of Epidemiology, vol. 126, no. 2, pp. 161–169, 1987. View at Google Scholar
  48. S. V. Singh, A. V. Singh, P. K. Singh, J. S. Sohal, and N. P. Singh, “Evaluation of an indigenous ELISA for diagnosis of Johne's disease and its comparison with commercial kits,” Indian Journal of Microbiology, vol. 47, no. 3, pp. 251–258, 2007. View at Publisher · View at Google Scholar
  49. P. K. Singh, S. V. Singh, A. V. Singh, and J. S. Sohal, “Screening of tissues and serum by culture, PCR and ELISA for the detection of Mycobacterium avium subspecies paratuberculosis from cases of clinical ovine Johne's disease in farmer's flocks,” Indian Journal of Animal Sciences, vol. 78, no. 10, pp. 1052–1056, 2008. View at Google Scholar
  50. W. B. McNab, A. H. Meek, J. R. Duncan et al., “An evaluation of selected screening tests for bovine paratuberculosis,” Canadian Journal of Veterinary Research, vol. 55, no. 3, pp. 252–259, 1991. View at Google Scholar
  51. R. J. Whittington and E. Sergeant, “Progress towards understanding the spread, detection and control of Mycobacterium avium subsp paratuberculosis in animal populations,” Australian Veterinary Journal, vol. 79, no. 4, pp. 267–278, 2001. View at Google Scholar