Diagnostic Application of IS900 PCR Using Blood as a Source Sample for the Detection of Mycobacterium avium Subspecies Paratuberculosis in Early and Subclinical Cases of Caprine Paratuberculosis

Table 2

Comparison of blood-PCR with ELISA (type I and type II reactors) and microscopic examination for the detection of Mycobacterium avium subsp. Paratuberculosis infection in kids.

Tests	Combinations
	1	2	3	4	5	6	7	8
PCR	$+$	$-$	$+$	$-$	$-$	$+$	$-$	$+$
ME	$+$	$-$	$-$	$+$	$-$	$+$	$+$	$-$
ELISA	$+$	$-$	$-$	$-$	$+$	$-$	$+$	$+$
Total (A)	1	2	4	2	1	11	0	0
Total (B)	6	2	1	0	1	6	2	3

(A) represent the total number of positive animals in different diagnostic combinations and type I reactor was considered as positive in ELISA. (B) represent the total number of positive animals in different diagnostic combinations and type II reactor was considered as positive in ELISA.