Research Article

The In Vitro Effects of Aqueous and Ethanolic Extracts of the Leaves of Ageratum conyzoides (Asteraceae) on Three Life Cycle Stages of the Parasitic Nematode Heligmosomoides bakeri (Nematoda: Heligmosomatidae)

Table 2

Mean mortality rate of Heligmosomoides bakeri L1 and L2 larvae incubated in increasing concentrations (mg·mL−1) of aqueous and ethanolic extracts of Ageratum conyzoides compared to Mebendazole.

Larval stagesTreatmentNegative controlsTested concentrations (mg·mL−1)
Distilled water5% tween0.6251.252.53.75

L1 larvaeAqueous extract3.5 ± 5.0NA6.0 ± 5.18.1 ± 1.032.3 ± 12.455.0 ± 22.3
Ethanolic extractNA3.0 ± 4.86.7 ± 4.560.7 ± 6.896.3 ± 4.899.4 ± 1.2
Mebendazole3.5 ± 5.0NA33.8 ± 21.132.2 ± 12.489.4 ± 7.484.4 ± 3.8

L2 larvaeAqueous extract3.5 ± 5.0NA32.6 ± 20.3440.8 ± 15.050.1 ± 13.158.2 ± 20.0
Ethanolic extractNA3.0 ± 4.813.6 ± 7.032.5 ± 4.235.4 ± 29.095.5 ± 5.2
Mebendazole3.5 ± 5.0NA77.0 ± 3.586.7 ± 5.296.0 ± 0.8100.0 ± 0.0

NA: not applicable.