Oocyte Source and Hormonal Stimulation for In Vitro Fertilization Using Sexed Spermatozoa in Cattle
Figure 1
Weekly scheme and hormone treatment for Ovum pick-up (OPU). (a) The animals were not treated with hormone FSH. Transvaginal OPUs (triangle) were performed on Monday and Thursday of each week for consecutive two weeks, resulting in a total of four sessions. (b) FSH treatment: FSH (arrow) at dosage of 80 mg IM and 120 SC was simultaneously administered on Day 1 (Saturday). Ovum pick-ups were performed 48 and 96 hours post FSH injection, meaning on Day 3 and Day 5 of each week for consecutive two weeks, resulting in a total of four sessions.
