Diagnosis and Molecular Characterization of Mycobacterium avium subsp. paratuberculosis from Dairy Cows in Colombia

Table 2

Enzyme-linked immunosorbent assay (ELISA), polymerase chain reaction (PCR) and fecal culture positive results of five dairy herds examined for Map in Colombia.

Herd	District	Herd cattle population	Number of samples^a	Serum ELISA-C	Fecal culture^c	Fecal PCR^e
1	Monterredondo	125	75	2	4^d	2
2	El Yuyal	174	53^b	4	0	0
3	El Yuyal	144	84	0	0	ND^f
4	Santo Domingo	172	94	0	0	ND
5	Santa Bárbara	38	23	0	0	ND
Total		653	329	6	4	2

^aOnly cows over 2 years of age were sampled.
^bIn this herd, 110 animals were sampled for feces, but only 53 of them were sampled for serum.
^cRefers to fecal samples cultured individually.
^dPositive results of one lymph node, one colon tissue, and two slurry samples are not included.
^eRefers to individual fecal samples part of pooled fecal samples positive by culture, and to fecal samples of positive ELISA-C animals tested individually.
^fND not done.