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Veterinary Medicine International
Volume 2011, Article ID 670987, 7 pages
Research Article

Ultrastructure of Sheep Primordial Follicles Cultured in the Presence of Indol Acetic Acid, EGF, and FSH

1Universidade Estadual de Londrina, DCV—CCA—UEL, 86051-990, Londrina, PR, Brazil
2University of Copenhagen, Groennegaardsvej 7, 1870 Frederiksberg C, Denmark
3Departamento de Reprodução Animal, Universidade Estadual de São Paulo, 18610-307 Botucatu, SP, Brazil
4Universidade Federal do Ceará, 60020-181 Sobral, CE, Brazil
5Medicina Veterinária, Universidade Estadual do Ceará, 60740-000 Fortaleza, CE, Brazil

Received 31 May 2010; Revised 6 September 2010; Accepted 5 November 2010

Academic Editor: Ali Honaramooz

Copyright © 2011 Evelyn Rabelo Andrade et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The aim of this study was to investigate the ultrastructural characteristics of primordial follicles after culturing of sheep ovarian cortical slices in the presence of indol acetic acid (IAA), Epidermal Growth Factor (EGF), and FSH. To evaluate ultrastructure of primordial follicles cultured in MEM (control) or in MEM containing IAA, EGF, and FSH, fragments of cultured tissue were processes for transmission electron microscopy. Except in the control, primordial follicles cultured in supplemented media for 6 d were ultrastructurally normal. They had oocyte with intact nucleus and the cytoplasm contained heterogeneous-sized lipid droplets and numerous round or elongated mitochondria with intact parallel cristae were observed. Rough endoplasmic reticulum (RER) was rarely found. The granulosa cells cytoplasm contained a great number of mitochondria and abundant RER. In conclusion, the presence of IAA, EGF, and FSH helped to maintain ultrastructural integrity of sheep primordial follicles cultured in vitro.