Table 3: Specificity and sensitivity of isotypes/PPA-ELISAs.

PPASpecificitySensitivity
ELISASubclinicallyClinicallyTotal

IgG100.0%23.1%92.9%47.5%
IgG1 94.7%11.5%57.1%27.5%
IgG2 92.1%53.8%85.7%65.0%

The specificity of isotypes/PPA-ELISAs was calculated as % of cattle from the healthy control group testing negative. The sensitivity of each test was estimated as % of infected cattle (subclinically infected, clinically infected, or both) testing positive.