Differential Gene Expression Segregates Cattle Confirmed Positive for Bovine Tuberculosis from Antemortem Tuberculosis Test-False Positive Cattle Originating from Herds Free of Bovine Tuberculosis
Figure 3
The relative gene expression levels compared with the reference pool of RNA from healthy cattle, as determined by qPCR assays, for the single antemortem test-false positive (SFP, shaded box), double antemortem test-false positive (DFP, solid box), and bTB-infected (bTB, clear box) groups of cattle. Gene expression levels (in log2 fold change) were calculated using the published mathematical algorithm [37] in which the reference pool of RNA was set as baseline (0 value at -axis) and used as the calibrator. Statistically significant differences were determined using Student’s t-test and are shown at (*) and (**). The error bars represent the standard error of the mean expression level for a group of cattle. (a) Genes with increased expression in all groups of cattle. (b) Genes with decreased expression in all groups of cattle.
