TY - JOUR A2 - Kass, Philip H. AU - Ismail, A. A. AU - Wagner, S. AU - Murua Escobar, H. AU - Willenbrock, S. AU - Sterenczak, K. A. AU - Samy, M. T. AU - Abd El-Aal, A. M. AU - Nolte, I. AU - Wefstaedt, P. PY - 2012 DA - 2012/02/08 TI - Effects of High-Mobility Group A Protein Application on Canine Adipose-Derived Mesenchymal Stem Cells In Vitro SP - 752083 VL - 2012 AB - Multipotency and self-renewal are considered as most important features of stem cells to persist throughout life in tissues. In this context, the role of HMGA proteins to influence proliferation of adipose-derived mesenchymal stem cell (ASCs) while maintaining their multipotent and self-renewal capacities has not yet been investigated. Therefore, extracellular HMGA1 and HMGA2 application alone (10–200 ng/mL) and in combination with each other (100, 200 ng/mL each) was investigated with regard to proliferative effects on canine ASCs (cASCs) after 48 hours of cultivation. Furthermore, mRNA expression of multipotency marker genes in unstimulated and HMGA2-stimulated cASCs (50, 100 ng/mL) was analyzed by RT-qPCR. HMGA1 significantly reduced cASCs proliferation in concentrations of 10–200 ng/mL culture medium. A combination of HMGA1 and HMGA2 protein (100 and 200 ng/mL each) caused the same effects, whereas no significant effect on cASCs proliferation was shown after HMGA2 protein application alone. RT-qPCR results showed that expression levels of marker genes including KLF4, SOX2, OCT4, HMGA2, and cMYC mRNAs were on the same level in both HMGA2-protein-stimulated and -unstimulated cASCs. Extracellular HMGA protein application might be valuable to control proliferation of cASCs in context with their employment in regenerative approaches without affecting their self-renewal and multipotency abilities. SN - 2090-8113 UR - https://doi.org/10.1155/2012/752083 DO - 10.1155/2012/752083 JF - Veterinary Medicine International PB - Hindawi Publishing Corporation KW - ER -