Interaction of Bordetella bronchiseptica and Its Lipopolysaccharide with In Vitro Culture of Respiratory Nasal Epithelium
(a) Tissue culture exposed to B. bronchiseptica for 4 hours. Dead epithelial cells are indicated (arrow); in some cases cell death affected several cells forming intraepithelial cysts. A 40x magnified semithin section is shown. (b) Fetal rabbit nasal septum respiratory epithelium exposed to B. bronchiseptica for 4 hours. Increased goblet cell activity; besides the increased number of these cells, their location at different heights within the epithelium is obvious and some GC have partially protruded over the ciliated cells and released their content (M). Cytoplasmic vacuolation of other epithelial cells (arrows) and a dead cell are indicated (arrow head). A 100x magnified semithin section is shown. (c) The effect of B. bronchiseptica on nasal septum respiratory epithelium compared to tissues not exposed to the bacterium: dead cells (DC), desquamated cells (Des), vacuolated cells (Vac), goblet cell activity (GC), and infiltration of PMN (PMN) are indicated. Bars with different letters have statistical difference with significance level of 95% (). (d) Control fetal rabbit nasal septum respiratory epithelium tissue culture at 0 hours. A 100x magnified semithin section is shown. (e) Fetal rabbit nasal septum respiratory epithelium exposed to B. bronchiseptica. Positive staining of ciliated border is indicated (arrow). A 100x magnified IIP is shown. (f) Negative control fetal rabbit nasal septum respiratory epithelium not exposed to B. bronchiseptica. A 100x magnified IIP is shown.