Figure 6: Effect of refrigeration on asparaginase enzyme activity. There is no significant difference in asparaginase activity between refrigeration and freshly reconstituted drug or with duration of refrigeration, . As expected, asparaginase concentration has a significant effect on enzyme activity ( ), but no difference between cold storage types at any concentration tested. This verifies that refrigerated drug retains enzyme activity at 7 and 14 days similar to freshly prepared drug.