E. coli-Derived L-Asparaginase Retains Enzymatic and Cytotoxic Activity In Vitro for Canine and Feline Lymphoma after Cold Storage

Figure 8

Effect of freeze/thaw on asparaginase enzyme activity. The number of freeze/thaw cycles accounts for a minor portion of the statistical variance between cold storage groups. Post hoc tests did not identify a significant difference between any cold storage type compared to control. This verifies that frozen stocks retained enzyme activity with freeze-thaw cycles similar to freshly prepared drug.