Articular Cartilage Gene Expression after Coxofemoral Joint Luxation in the Dog

Table 1

Sequences of sense and antisense primers used for amplification in real-time PCR.

Gene	Accession number	Primer sequence (5′→ 3′)	Temp. °C	Amplicon size (bp)
AGG	U65989_2	Rw: ACTGCTCCAGGCGTGTGATG; Fw: GACCATGTCGTGCAGGTGAC	58	405
BAX	NM_001003011	Rw: TGCTGGCAAAGTAGAAGAGGGCAA; Fw: TTCCGAGTGGCAGCTGAGATGTTT	60	79
BCL-2	NM_001002949	Rw: GTGCTTTGCATTCTTGGATGAGGG; Fw: CATGCCAAGAGGGAAACACCAGAA	62	76
CAS-3	NM_001003042	Rw: TTCTGACAGGCCATGTCATCCTCA; Fw: TTCATTATTCAGGCCTGCCGAGG	62	83
CAS-9	NM_001031633	Rw: TTGTTGATGATGAGGCAGTAGCCG; Fw: TCAGTGACGTCTGTGTTCAGGAGA	61	97
COL2A1	AF023169	Rw: TGCTTTCCAGTTGGGCCAGC; Fw: GAGCTCCTGGTGCATCTGGA	58	233
MMP-3	AY183143_1	Rw: CAGAGCTTTCTCAATGGCAG; Fw: CTCACCCAGCAATACCTAGA	55	297
GAPDH	DQ403060	Rw: CGAAGTGGTCATGGATGACT; Fw: AGTATGATTCTACCCACGGC	55	362
HAS-1	XM_849398	Rw: GCATAGAAGAGCCGCAACAC; Fw: CAGACACGCTGGTCCAAATC	55	149
HAS-2	XM_539153	Rw: GACTCATCCGTCTCACCAG; Fw: GGTCATAGATGGGAACTCG	51	135
TIMP-1	AF077817_1	Rw: TGTCACTCTGCAGTTTGCAG; Fw: GATGTTCAAGGGTTTCAGCG	55	294