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Veterinary Medicine International
Volume 2014, Article ID 892421, 6 pages
http://dx.doi.org/10.1155/2014/892421
Research Article

Electrophoretic Analysis of Indian Isolates of Mycoplasma agalactiae and Mycoplasma bovis by SDS-PAGE and Immunoblotting

1Department of Microbiology, Uttar Pradesh Pandit Deen Dayal Upadhayay Pashu Chikitsa Vigyan Vishwa Vidyalaya Evam Go-Anusandhan Sansthan (DUVASU), Mathura 281001, India
2National Referral Laboratory on Mycoplasma, Division of Bacteriology & Mycology, Indian Veterinary Research Institute, Izzatnagar 243122, India
3Division of Bacteriology & Mycology, Indian Veterinary Research Institute, Izzatnagar 243122, India
4Divisionof Animal Science, Central Agricultural Research Institute, Port Blair, A & N Islands 744101, India

Received 27 December 2013; Revised 1 March 2014; Accepted 17 March 2014; Published 3 April 2014

Academic Editor: Praveen Malik

Copyright © 2014 Amit Kumar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Mycoplasma agalactiae and Mycoplasma bovis both are responsible for respiratory conditions in sheep and goats. M. agalactiae is a major pathogen of sheep and goats and accounts for almost 90% of outbreaks of contagious agalactia syndrome in goats and almost 100% in sheep. On the basis of clinical signs and cultural, morphological, and biochemical characterization it is almost impossible to differentiate between both the species. Moreover, due to presence of genomic and proteomic similarity most of the time routine diagnostic tests fail to differentiate between them. Hence the present study was conducted to find out the protein profile of isolates of both the species by SDS-PAGE and to find out the cross-reacting as well as differentiating immunogenic proteins by Immunoblotting, which can be of immunoprophylactic as well as diagnostic values. The study revealed 6-7 major immunogenic cross-reactive proteins with the presence of two important non-cross-reacting species specific polypeptides particularly 25.50 and 24.54 kDa in M. agalactiae and M. bovis, respectively, that might be of diagnostic values.