In Vitro Virucidal and Virustatic Properties of the Crude Extract of Cynodon dactylon against Porcine Reproductive and Respiratory Syndrome Virus
Figure 1
Virustatic activity of C. dactylon study reagent on replication of porcine reproductive and respiratory syndrome virus (PRRSV). MARC-145 cells were infected with PRRSV at a multiplicity of infection of 1. At various time points after infection, PRRSV in supernatants were quantified for virus titer by immunoperoxidase monolayer assay. In control group, viruses in supernatants at 24 hpi were infected and replicated in the MARC-145 cells (a), while little or no PRRSV were observed in groups of study reagent (b, c). At the concentration of 0.78 mg/mL, virus could only be observed in supernatants of 48 hpi suggesting that PRRSV growth was interrupted by C. dactylon. Representative data are mean and standard error. Asterisks indicate statistical significance ( value ≤ 0.05). Scale bar in (a, b, and c) ≈180 μm.
