Research Article

Comparison of the Effect of Two Purification Methods on the Immunogenicity of Recombinant Outer Membrane Protein H of Pasteurella multocida Serovar A:1

Table 1

Experimental design and results of P. multocida challenge of immunized and nonimmunized chickens.

Treatment
groups
Type of immunogenP. multocida strain challenge subgroup Number of survivors/total (% protection)
X-73P-1059

1rOmpH purified using electroelution 9/10 (90)8/10 (80)
2rOmpH purified using a denatured condition of affinity chromatography2/10 (20)3/10 (30)
3Native OmpH purified using electroelution10/10 (100)10/10 (100)
4Incomplete Freund’s adjuvant0/5 (0)0/5 (0)
5No immunization0/5 (0)0/5 (0)

Significantly difference ().